Our company's research and development platform is single B-cell sorting technology, used for the research, development, and production of rabbit monoclonal antibodies. It uses rabbits as immunization animals, utilizes single B-cell sorting platforms, combined with high-throughput screening technology, to screen for specific positive B-cell clones. Through PCR technology, we clone the heavy and light chains of recombinant rabbit monoclonal antibodies, obtain monoclonal supernatants through TAP technology transfection, and after screening and testing, conduct large-scale production. We can prepare monoclonal antibodies with high specificity and high affinity for use in scientific research, disease diagnosis, drug development, and other fields to meet customer needs.
Custom Services
Service Process
Service Advantages
1. High throughput: Can perform high-throughput screening of single B cells.
2. Short cycle: From sorting to obtaining monoclonal antibody supernatant available for sample verification, it only takes 2 weeks.
3. Comprehensive validation: Can perform detection validation in various applications (IHC, IF, ICC, IP, ChIP, WB, etc.) in multiple tissue/cell samples according to customer needs.
Service Content and Description
| Standard Process | Steps | Period | Deliverables | QC Standard |
|---|---|---|---|---|
| Antibody Preparation Design | Protein sequence analysis, antigen design | 1 working day | Antigen sequence | N/A |
| Polypeptide Immunogen Preparation | Polypeptide synthesis | 2-3 weeks | / | MS&HPLC |
| Polypeptide conjugation to carrier protein | 1 week | / | N/A | |
| Protein Immunogen Preparation | Codon optimization, gene synthesis, plasmid construction | 2-3 weeks | Sequencing report | Correct sequencing |
| Protein expression and purification | 2-3 weeks | / | SDS-PAGE | |
| Animal Immunization, Serum Evaluation | Immunize 2-3 rabbits, collect serum for titer detection | 8-12 weeks | Qualified antiserum sample for testing | Indirect ELISA |
| Single B Cell Sorting | / | 1 week | / | N/A |
| Sequence Retrieval, Cloning, Small-scale Antibody Expression | Retrieve antibody variable region sequences from sorted single B cells, recombinantly express antibody samples | 4-6 weeks | Small-scale transfection cell supernatant | Indirect ELISA |
| Antibody Scale-up Production | Construct antibody variable region sequences into plasmids, transfect eukaryotic cells | 2 weeks | / | |
| Antibody Purification | Collect transfected eukaryotic cell supernatant, purify antibodies | 2 weeks | Purified antibodies | Indirect ELISA |
| Report Preparation, Project Conclusion | Organize report | 1 week | Project conclusion report | N/A |
Customer Provides
1. Fill out the complete project information collection form, including information such as the species and sequence of the target gene.
2. Customer provides protein antigen: protein purity greater than 85%, protein concentration greater than 0.5mg/mL, 3-5mg purified protein.
3. Customer provides expression plasmid: plasmid concentration greater than 100ng/μL, not less than 10μL (must provide a sequencing report confirming the correct plasmid construction).
4. Customer provides small molecule antigen: need to provide the specific small molecule chemical structural formula or CAS search number to confirm whether there are available chemical groups for carrier protein conjugation.
Final Delivery
1. Purified antibodies: 1-2 clones of recombinant production antibodies, 2-5mg per clone.
2. Antibody sequences: variable region sequences of 1-2 clones.
3. Complete project conclusion report.
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