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HUABIO Custom Polyclonal Antibody Services

Custom Polyclonal Antibody Services

Service Items Time (weeks) Peptide package Peptide package Peptide package (Methyl-specific/acetyl-specific) Protein package/Purified protein Protein package/Expression plasmid Protein package/Gene template
Peptide antigen
Peptide synthesis 1~2
Peptide conjugation to KLH
Protein antigen
Vector construction 2~4
Protein expression & purification
Immunization
Immunization of 2 rabbits 8~10
ELISA detection
Purification and detection
Affinity purification 1
Antigen-specific affinity purification
ELISA detection 1
Total time (weeks) 11~13 9~10 10~12 11~12 11~12

Deliverables

Remaining antigen provided free of charge (for client's own antibody testing purposes).

Polyclonal antibody serum (unpurified).

1–10 mg of purified antibody, with ELISA titer ≥1:64,000; any remaining purified antibody and serum will belong to the client.

Comprehensive reports, detailing immunization, testing, purification, and ELISA detection.

Advantages of Polyclonal Antibodies:

1. Diverse Immunization Hosts

We offer immunization services using various animal hosts such as rabbits, rats, mice, guinea pigs, and goats, satisfying diverse customer requirements for species-specific antibody sources.

2. Optimal Antigen Design

Utilizing intelligent antigen-design software combined with over 15 years of antibody production experience ensures higher success rates in obtaining qualified antibodies suitable for applications.

3. Wide Variety of Antigen Types

We can prepare antibodies against diverse antigen types, including conventional peptides (with various modifications such as phosphorylation, acetylation, methylation, ubiquitination, glycosylation, lactylation, crotonylation, succinylation, hydroxyproline modifications, etc.), protein antigens, and small molecule antigens.

4. Flexible Immunization Schedule and High Antibody Titers

We adjust immunization cycles according to different antigen types to rapidly achieve qualified antibody titers. We guarantee an ELISA OD450 nm value ≥0.6 at a dilution of 1:64,000, with achievable titers exceeding 1:256,000.

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