Monoclonal antibodies (mAbs) are fully homogeneous antibodies produced by a single B-cell clone that recognize only a single specific antigenic epitope. Huabio provides a one-stop solution from antigen preparation to final cell line and antibody delivery. Through mature cell fusion technology, multiple rounds of strict screening and single-cell cloning processes, we ensure that each hybridoma cell line can stably secrete a single antibody targeting the antigenic epitope you need. The delivered hybridoma cell line will become a valuable biological resource that you can inherit permanently, fundamentally solving the problems of antibody supply interruption and batch-to-batch differences.
Huabio is committed to developing high-quality monoclonal antibodies with high specificity, high affinity, and excellent batch consistency for your basic scientific research, in vitro diagnostic (IVD) reagent development, and therapeutic antibody discovery.
Custom Services
Service Process
Service Advantages
1. Nearly 20 years of antigen design experience, improving project success rate by optimizing antigen technical routes that better meet customer application needs.
2. From antigen design consultation, immunization program formulation to final antibody validation, our expert team provides full professional technical support and transparent progress feedback, ensuring excellence in every link and saving you valuable time and energy.
3. According to your specific needs, we can flexibly customize immunogens (proteins, polypeptides, cells, etc.), screening strategies (such as functional screening using natural proteins or specific cells), and antibody application validation programs to ensure that the obtained antibodies perfectly fit your project goals.
Service Content and Description
| Standard Process | Steps | Period | Deliverables | QC Standard |
|---|---|---|---|---|
| Antibody Preparation Design | Protein sequence analysis, antigen design | 1 working day | Antigen sequence | N/A |
| Polypeptide Immunogen Preparation | Polypeptide synthesis | 2-3 weeks | / | MS&HPLC |
| Polypeptide conjugation to carrier protein | 1 week | / | N/A | |
| Protein Immunogen Preparation | Codon optimization, gene synthesis, plasmid construction | 2-3 weeks | Sequencing report | Correct sequencing |
| Protein expression and purification | 2-3 weeks | / | SDS-PAGE | |
| Animal Immunization, Serum Evaluation | Immunize 5 mice, collect serum for titer detection | 6-7 weeks | Qualified antiserum sample for testing | Indirect ELISA |
| Cell Fusion, Monoclonal Screening | Fusion, multiple rounds of subcloning screening | 4-5 weeks | / | Indirect ELISA |
| Cell Supernatant Collection | Collect cell supernatant from screened monoclonals and freeze cells. | 2-3 weeks | 5-20 monoclonal cell line supernatants | Indirect ELISA |
| Ascites Preparation, Antibody Purification | Client-validated best clone, perform ascites induction production and antibody purification | 3-4 weeks | Purified antibodies | Indirect ELISA |
| Report Preparation, Project Conclusion | Organize report | 1 week | Project conclusion report | N/A |
Customer Provides
1. Fill out the complete project information collection form, including information such as the species, sequence, and application of the target gene.
2. Customer provides protein antigen: protein purity greater than 85%, protein concentration greater than 0.5mg/mL, 3-5mg purified protein.
3. Customer provides polypeptide antigen: purity greater than 85%, no less than 8 amino acids, at least 10mg of naked peptide.
4. Customer provides expression plasmid: plasmid concentration greater than 100ng/μL, not less than 10μL (must provide a sequencing report confirming the correct plasmid construction).
5. Customer provides small molecule antigen: need to provide the specific small molecule chemical structural formula or CAS search number to confirm whether there are available chemical groups for carrier protein conjugation.
Final Delivery
1. Purified antibodies: Deliver 1-2 clones of purified antibodies, 5-10mg per clone.
2. Cell lines: Provide 2-5 positive monoclonal cell lines according to customer requirements;
3. Report: Deliver a complete project report (including experimental procedures, screening and testing data).
Note: If you need to obtain antibody sequences, you can additionally entrust us to retrieve antibody sequences from monoclonal cell lines, and the cost needs to be consulted separately.
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