HUABIO Custom Mouse Monoclonal Antibody Services
HUABIO Custom Mouse Monoclonal Antibody Services
We Provide:
- Purified antibodies: 5–10 mg per clone
- Cell lines: 2 positive cell lines provided per client request
- Report: Comprehensive project process and experimental results report
| Service Items | Time (weeks) | Peptide package | |||
|---|---|---|---|---|---|
| Peptide | Purified protein | Expression plasmid | Gene template | ||
| Peptide antigen | 2~3 | √ | |||
| Peptide conjugation to KLH | √ | ||||
| Protein antigen | 2~4 | ||||
| Vector construction | √ | ||||
| Protein expression and purification | √ | √ | |||
| immunization(5 mice) | 8~10 | √ | √ | √ | √ |
| Elisa & WB detection | |||||
| Fusion & Screening | 2 | √ | √ | √ | √ |
| Subcloning(5 plates) | 2 | √ | √ | √ | √ |
| Ascites production(Injection on 5 mice) | 4 | √ | √ | √ | √ |
| Subtype Identification & Purification | 1 | √ | √ | √ | √ |
| Total time (weeks) | 19~22 | 17~19 | 19~21 | 21~23 | |
Process Features:
Short Production Cycle: The entire cycle—from immunization initiation to completion of the first subcloning—takes approximately 60 days.
Antigen Saving: Requires only 3–5 mg of protein or peptide antigen.
Flexible Process: Our rapid mouse monoclonal antibody preparation technology is interchangeable with classical mouse monoclonal antibody preparation techniques under suitable conditions, without affecting subsequent production steps or antibody quality.
Service Details:
Antigen Preparation: 3–5 mg of protein antigen or peptide antigen.
Mouse Immunization: Immunization of 5 BALB/c mice per project.
Cell Fusion: Fusion using optimized SP2/0 myeloma cells originally sourced from ATCC, USA.
Antibody Preparation Cycle: ≥2 months.
Customizable Product Types and Pricing Options available for clients.