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Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein

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Catalog# HA210202

Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein

Product Information

Product name

Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein

Catalog No.

HA210202

Predicted molecular mass

The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

Protein construction description

Recombinant Human HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and RMFPNAPYL peptide.

Accession

A0A140T913(HLA-A*02:01)&P61769(B2M)&RMFPNAPYL

Source

HEK293

Purity

> 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC

Endotoxin

Less than 1EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Species

Human

Background

The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of  the MHC Class I, B2M and RMFPNAPYL peptide of  the WT-1.

Usage guide

Storage

-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

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