ELKS Recombinant Rabbit Monoclonal Antibody [PSH17-33]
Overview
Product Name
ELKS Recombinant Rabbit Monoclonal Antibody [PSH17-33]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human ELKS aa 1-450.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 128 kDa
Positive Control
HeLa cell lysate, Huh7 cell lysate, U-87 MG cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Mouse testis tissue lysate, Mouse kidney tissue lysate, Rat brain tissue lysate, Rat testis tissue lysate, Rat kidney tissue lysate.
Conjugation
unconjugated
Clone Number
PSH17-33
Product Features
Form
Liquid
Concentration
1 mg/mL.
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
Target
Function
ELKS/RAB6-interacting/CAST family member 1 is a protein that in humans is encoded by the ERC1 gene. The name ELKS is derived from "protein rich in the amino acids E, L, K and S" The protein encoded by this gene is a member of a family of RIM-binding proteins. RIMs are active zone proteins that regulate neurotransmitter release. This gene has been found fused to the receptor-type tyrosine kinase gene RET by gene rearrangement due to the translocation t(10;12)(q11;p13). Multiple transcript variants encoding different isoforms have been found for this gene. ELKS has been reported to direct vesicles with RAB6A to melanosomes.
Background References
1. Ghelani T et al. Interactive nanocluster compaction of the ELKS scaffold and Cacophony Ca(2+) channels drives sustained active zone potentiation. Sci Adv. 2023 Feb
2. Liang M et al. Oligomerized liprin-alpha promotes phase separation of ELKS for compartmentalization of presynaptic active zone proteins. Cell Rep. 2021 Mar
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Membrane, Golgi apparatus membrane, Presynaptic cell membrane, Cell projection, podosome.
Synonyms
Cast 2 antibody
CAST2 antibody
CAZ associated structural protein 2 antibody
Elks antibody
ELKS/RAB6 interacting/CAST family member 1 antibody
ELKS/RAB6-interacting/CAST family member 1 antibody
ERC 1 antibody
ERC protein 1 antibody
ERC-1 antibody
Erc1 antibody
ExpandImages
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Western blot analysis of ELKS on different lysates with Rabbit anti-ELKS antibody (HA723916) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Huh7 cell lysate (20 µg/Lane)
Lane 3: U-87 MG cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: Neuro-2a cell lysate (20 µg/Lane)
Lane 6: PC-12 cell lysate (20 µg/Lane)
Lane 7: C6 cell lysate (20 µg/Lane)
Lane 8: Mouse brain tissue lysate (40 µg/Lane)
Lane 9: Mouse testis tissue lysate (40 µg/Lane)
Lane 10: Mouse kidney tissue lysate (40 µg/Lane)
Lane 11: Rat brain tissue lysate (40 µg/Lane)
Lane 12: Rat testis tissue lysate (40 µg/Lane)
Lane 13: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 128 kDa
Observed band size: 120/130 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723916) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
ELKS Recombinant Rabbit Monoclonal Antibody [PSH17-33] - BSA and Azide free
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
