Nrf3 Rabbit Polyclonal Antibody
Catalog# HA500570
Nrf3 Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
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unconjugated
Overview
Product Name
Nrf3 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Mouse Nrf3 aa 1-100.
Species Reactivity
Human, Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 73 kDa
Positive Control
HeLa cell lysate, 3T3-L1 cell lysate, Mouse placenta tissue lysate.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
2 mg/mL.
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
Nuclear factor (erythroid 2)-like factor 3, also known as NFE2L3 or 'NRF3', is a transcription factor that in humans is encoded by the Nfe2l3 gene. Nrf3 is a basic leucine zipper (bZIP) transcription factor belonging to the Cap ‘n’ Collar (CNC) family of proteins. The specific functions of the NRF3 protein are still unknown, but some putative functional properties have been inferred from those of NFE2L1 due to their structural similarity. It is known that NRF3 can heterodimerize with small musculo-aponeurotic fibro-sarcoma (MAF genes) factors to bind antioxidant response elements in target genes.
Background References
1. Gurri S et al. NRF3 suppresses squamous carcinogenesis, involving the unfolded protein response regulator HSPA5. EMBO Mol Med. 2023 Nov
2. Hirose S et al. NRF3 activates mTORC1 arginine-dependently for cancer cell viability. iScience. 2023 Jan
Subcellular Location
Nucleus.
Synonyms
Nuclear factor erythroid 2-related factor 3
NF-E2-related factor 3
NFE2-related factor 3
Nuclear factor, erythroid derived 2, like 3
Nfe2l3
Nrf3
Images
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Western blot analysis of Nrf3 on different lysates with Rabbit anti-Nrf3 antibody (HA500570) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 3T3-L1 cell lysate
Lane 3: Mouse placenta tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 73 kDa
Observed band size: 73 kDa
Exposure time: Lane 1: 10 seconds; Lane 2: 28 seconds; Lane 3: 46 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500570) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"