Mammal NF-L enzyme-linked immunoassay kit
Catalog# EH0154
Mammal NF-L enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
Standard curve range
-
6.86~5000 pg/ml
General Overview
Product Name
Mammal NF-L enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.54 pg/ml (50 μl)；1.31 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0058 | 0.0061 | 0.0060 | |
| 6.86 | 0.0192 | 0.0156 | 0.0174 | 0.0115 |
| 20.58 | 0.0364 | 0.0350 | 0.0357 | 0.0298 |
| 61.73 | 0.0947 | 0.0897 | 0.0922 | 0.0863 |
| 185.19 | 0.2678 | 0.2753 | 0.2716 | 0.2656 |
| 555.56 | 0.7753 | 0.7460 | 0.7607 | 0.7547 |
| 1666.67 | 2.0194 | 1.9554 | 1.9874 | 1.9815 |
| 5000.00 | 3.9390 | 3.9208 | 3.9299 | 3.9240 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 53.0 | 385.7 | 1612.4 | 127.7 | 547.7 | 1800.6 |
| Standard Deviation | 2.2 | 17.4 | 76.9 | 6.7 | 25.3 | 78.4 |
| Coefficient of Variation (%) | 4.1 | 4.5 | 4.8 | 5.2 | 4.6 | 4.4 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的哺乳动物 NF-L，未加哺乳动物 NF-L 的血清作为本底，计算回收率。回收率的范围从 96 % 至 104 %，平均回收率为 101%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-1.18 | 25 | 0.55 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Function
NF-L 是一种在神经元中表达的 68 kDa 轻链细胞骨架中间丝蛋白。它与 125 kDa NF-M 和 200 kDa NF-H 结合形成神经丝。