Specification
Catalog# ET1701-41
PD-L1 Recombinant Rabbit Monoclonal Antibody [JJ08-95]
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WB
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IHC-P
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Human
Overview
Product Name
PD-L1 Recombinant Rabbit Monoclonal Antibody [JJ08-95]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human PD-L1 aa 191-240 / 290.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 33 kDa
Positive Control
MDA-MB-231 cell lysate, A375 cell lysate, U-87 MG cell lysate, THP-1 cell lysate, human non-small cell lung cancer tissue.
Conjugation
unconjugated
Clone Number
JJ08-95
RRID
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-P
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1:50-1:200
Applications in Publications
Species in Publications
Human | See 7 publications below |
Dog | See 1 publications below |
Mouse | See 1 publications below |
Target
Function
Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T cell proliferation, cytokine production, differentiation of effector T cells, and the induction of Bcl-x, a promoter of T cell survival. Conversely, engagement of CTLA4 by B7-1 or B7-2 may inhibit proliferation and IL-2 production. Pdcd-1L1 (programmed cell death ligand-1), also known as B7-H1 or PD-L1, is 290 amino acid type I transmembrane protein which is 20% and 15% identical to B7-1 and B7-2, respectively. Pdcd-1L2 has immunoglobulin V-like and C-like domains and a 30 amino acid cytoplasmic tail. It does not bind CD28, cytotoxic T-lymphocyte A4 or ICOS (inducible co-stimulator). IL-2, although produced in small amounts, is required for the effect of Pdcd-1L1 co-stimulation. The gene which encodes Pdcd-1L1 maps to human chromosome 9p24. Pdcd-1L2 (programmed cell death ligand-2) is a 73 amino acid protein which contains a signal sequence, IgV- and IgC-like domains, a transmembrane region and a cytoplasmic region. The gene which encodes Pdcd-1L2 maps to human chromosome 9p24.2. The constitutive expression of Pdcd-1L1 and Pdcd-1L2 on paren-chymal cells of heart, lung and kidney suggests that the Pdcd-1-Pdcd-L system could provide unique negative signaling to help prevent autoimmune disease.
Background References
1. Jurado J.O., et al. 2008. Programmed death (PD)-1:PD-ligand 1/PD-ligand 2 pathway inhibits T cell effector functions during human tuberculosis. J. Immunol. 181:116-125.
2. Boorjian S.A., et al. 2008. T-cell coregulatory molecule expression in urothelial cell carcinoma: clinicopathologic correlations and association with survival. Clin. Cancer Res. 14:4800-4808.
Sequence Similarity
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Tissue Specificity
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Post-translational Modification
Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation.
Subcellular Location
Cell membrane, Early endosome membrane, Recycling endosome membrane, Nucleus.
UNIPROT
Synonyms
B7 H antibody
B7 H1 antibody
B7 homolog 1 antibody
B7-H1 antibody
B7H antibody
B7H1 antibody
CD 274 antibody
CD274 antibody
CD274 antigen antibody
CD274 molecule antibody
ExpandB7 H antibody
B7 H1 antibody
B7 homolog 1 antibody
B7-H1 antibody
B7H antibody
B7H1 antibody
CD 274 antibody
CD274 antibody
CD274 antigen antibody
CD274 molecule antibody
MGC142294 antibody
MGC142296 antibody
OTTHUMP00000021029 antibody
PD L1 antibody
PD-L1 antibody
PD1L1_HUMAN antibody
PDCD1 ligand 1 antibody
PDCD1L1 antibody
PDCD1LG1 antibody
PDL 1 antibody
PDL1 antibody
Programmed cell death 1 ligand 1 antibody
Programmed death ligand 1 antibody
RGD1566211 antibody
CollapseImages
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Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (ET1701-41) at 1/5,000 dilution.
Lane 1: MDA-MB-231 cell lysate
Lane 2: A375 cell lysate
Lane 3: U-87 MG cell lysate
Lane 4: THP-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 45 kDa
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-41) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer tissue using anti-PD-L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Identification of Treg-related prognostic molecular subtypes and individualized characteristics in clear cell renal cell carcinoma through single-cell transcriptomes and bulk RNA sequencing
Author: Weng Kangqiang,et al
PMID: 38442575
Journal: International Immunopharmacology
Application: IHC
Reactivity: Human
Publish date: 2024 Mar
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Citation
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Discovery of Momordin Ic that selectively reduces PD-L1 expression in multiple myeloma cells by recruiting SYVN1
Author: Wu Sizhi,et al
PMID: NOPMID20240723
Journal: Food Bioscience
Application: WB
Reactivity: Human
Publish date: 2024 Jul
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Citation
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Clinical activity and safety of sintilimab, bevacizumab, and TMZ in patients with recurrent glioblastoma
Author: Lu Yinghao,et al
PMID: 38273249
Journal: BMC Cancer
Application: IHC
Reactivity: Human
Publish date: 2024 Feb
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Citation
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Discovering New Metallo-Deubiquitinase CSN5 Inhibitors by a Non-Catalytic Activity Assay Platform
Author: Yan Yuhang,et al
PMID: 39129245
Journal: Journal Of Medicinal Chemistry
Application: WB
Reactivity: Human
Publish date: 2024 Aug
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Citation
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PD-1/PD-L1 Interaction Upregulates YAP1 Expression in HepG2 Cells Through MAPK/ERK Pathway
Author: Li Shenghao,et al
PMID: NO PMID20231001
Journal: Natural Product Communications
Application: WB
Reactivity: Human
Publish date: 2023 Oct
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Citation
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Reversing stage III oral adenocarcinoma in a dog treated with anti-canine PD-1 therapeutic antibody: a case report
Author:
PMID: 37252387
Journal: Frontiers In Veterinary Science
Application: WB
Reactivity: Dog
Publish date: 2023 May
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Citation
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Overexpression of POLA2 in hepatocellular carcinoma is involved in immune infiltration and predicts a poor prognosis
Author:
PMID: 37452331
Journal: Cancer Cell International
Application: WB
Reactivity: Human
Publish date: 2023 Jul
-
Citation
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Identification of a Hypoxia-Related Signature for Predicting Prognosis and the Immune Microenvironment in Bladder Cancer
Author:
PMID: 34026819
Journal: Frontiers In Molecular Biosciences
Application: IHC
Reactivity:
Publish date: 2021 May
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Citation
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BRAF Mutation as a Potential Therapeutic Target for Checkpoint Inhibitors: A Comprehensive Analysis of Immune Microenvironment in BRAF Mutated Colon Cancer
Author: Cen, S., Liu, K., Zheng, Y., Shan, J., Jing, C., Gao, J., Pan, H., Bai, Z., & Liu, Z.
PMID: 34381786
Journal: Frontiers In Cell And Developmental Biology
Application: IHC
Reactivity: Human
Publish date: 2021 Jul
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Citation
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Combination Foretinib and Anti-PD-1 Antibody Immunotherapy for Colorectal Carcinoma. Frontiers in cell and developmental biology, 9, 689727.
Author: Fu, Y., Peng, Y., Zhao, S., Mou, J., Zeng, L., Jiang, X., Yang, C., Huang, C., Li, Y., Lu, Y., Wu, M., Yang, Y., Kong, T., Lai, Q., Wu, Y., Yao, Y., Wang, Y., Gou, L., & Yang, J.
PMID: 34307367
Journal: Frontiers In Cell And Developmental Biology
Application: WB
Reactivity: Mouse
Publish date: 2021 Jul
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Citation
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