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Anti-idiotype antibodies are a class of antibodies that specifically recognize the variable region of another antibody molecule, whose mechanism of action is based on the idiotype-anti-idiotype network theory of antibody molecules. In scientific research, anti-idiotype antibodies can serve as important tools for disease model establishment, antigen epitope identification, and autoimmune disease mechanism research. In clinical applications, anti-idiotype antibodies show broad application prospects in vaccine development, cancer treatment, and autoimmune disease treatment. With rich antibody research and development experience and a professional technical team, Huabio provides high-quality custom anti-idiotype antibody services, including the preparation of rabbit polyclonal, mouse monoclonal, and rabbit monoclonal anti-idiotype antibodies.

Service Advantages

1. Advanced Antibody Preparation Platform

Relying on the HiMab® recombinant rabbit monoclonal antibody platform and traditional hybridoma technology platform, we can provide multiple anti-idiotype antibody preparation solutions to meet different customer needs.

2. Efficient Antibody Purification Process

Using multiple purification technologies such as affinity chromatography and ion exchange chromatography to ensure high purity and high specificity anti-idiotype antibodies.

3. Rich Project Experience

Successfully completed multiple custom anti-idiotype antibody projects, involving multiple research fields such as cancer and autoimmune diseases.


Anti-idiotype Antibody Types


Final Delivery

Rabbit Polyclonal Anti-idiotype Antibody

Rabbit polyclonal anti-idiotype antibodies have the advantages of high affinity, short preparation cycle, and low cost, suitable for preliminary research and screening.

Service Stage Step Description Period
1. Project Evaluation and Design Communicate with customers, understand needs, evaluate project feasibility, and design experimental plans. 1-2 weeks
2. Immunogen Preparation Prepare immunogen according to the antibody sequence or sample provided by customers. 2-3 weeks
3. Animal Immunization
  • Primary immunization
  • Booster immunization (3 times)
  • Immunization titer detection
  • Blood collection
8-10 weeks
4. Antibody Purification and Identification
  • Serum separation
  • Affinity chromatography purification
  • Antibody purity detection (SDS-PAGE)
  • Antibody titer detection (ELISA)
2-3 weeks
5. Project Summary and Delivery
  • Write project report
  • Deliver antibodies and related materials
1-2 weeks
Deliverables
  • 10mg or more purified antibody (concentration ≥1mg/ml)
Quality Assurance
  • Provide complete project report, including immunization scheme, purification process, quality control data, etc.

Mouse Monoclonal Anti-idiotype Antibody

Service Stage Step Description Period
1. Project Evaluation and Design Communicate with customers, understand needs, evaluate project feasibility, and design experimental plans. 1-2 weeks
2. Immunogen Preparation Prepare immunogen according to the antibody sequence or sample provided by customers. 2-3 weeks
3. Animal Immunization
  • Primary immunization
  • Booster immunization (4 times)
  • Immunization titer detection
  • Pre-fusion booster immunization
8-10 weeks
4. Cell Fusion and Screening
  • Fusion of spleen cells and myeloma cells
  • Positive clone screening (ELISA)
  • Subcloning (limiting dilution method)
  • Antibody specificity identification
  • Expansion culture of positive clones
6-8 weeks
5. Antibody Production and Purification
  • Ascites preparation or cell culture
  • Antibody purification
4-6 weeks
6. Antibody Identification and Quality Control
  • Antibody purity detection (SDS-PAGE)
  • Antibody titer detection (ELISA)
  • Antibody specificity identification
2-3 weeks
7. Project Summary and Delivery
  • Write project report
  • Deliver antibodies and related materials
  • Compile experiment data
  • Perform final quality control checks
  • Package antibodies and materials
  • Provide after-sales support
1-2 weeks
Deliverables
  • 5mg or more purified antibody (concentration ≥1mg/ml)
  • 2-3 positive hybridoma cell lines
  • Complete project report
  • Antibody quality inspection report (SDS-PAGE, ELISA, etc.)
  • Hybridoma cell culture manual
  • Antibody usage instructions
  • Remaining ascites or cell culture supernatant (if applicable)

Rabbit Monoclonal Anti-idiotype Antibody

Service Stage Step Description Period
1. Project Evaluation and Design Communicate with customers, understand needs, evaluate project feasibility, and design experimental plans. 1-2 weeks
2. Immunogen Preparation Prepare immunogen according to the antibody sequence or sample provided by customers. 2-3 weeks
3. Animal Immunization
  • Primary immunization
  • Booster immunization (3 times)
  • Immunization titer detection
  • Blood collection
8-10 weeks
4. Single B Cell Screening and Cloning
  • Peripheral blood lymphocyte separation
  • Single B cell screening (FACS)
  • Single B cell culture and cloning
4-6 weeks
5. Antibody Expression, Purification and Identification
  • Antibody gene cloning and expression
  • Antibody purification
  • Antibody purity detection (SDS-PAGE)
  • Antibody titer detection (ELISA)
  • Antibody specificity identification
  • Antibody affinity determination (SPR)
  • Antibody stability detection
  • Antibody function verification (if applicable)
  • Project summary and delivery
6-8 weeks
Deliverables
  • 5mg or more purified antibody (concentration ≥1mg/ml)
  • Antibody gene sequence (if applicable)
  • Complete project report
  • Antibody quality inspection report (SDS-PAGE, ELISA, SPR, etc.)
  • Antibody usage instructions
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