CD11c Recombinant Rabbit Multiclonal Antibody [PSH13-25]

☑ Relative expression (RE)

Western blot analysis of CD11c on different lysates with Rabbit anti-CD11c antibody (HA723512) at 1/5,000 dilution.

Lane 1: THP-1 cell lysate
Lane 2: THP-1 starved for 2 hours then add 100ng/mL TPA for 48 hours cell lysate
Lane 3: RAW264.7 cell lysate (20 µg/Lane)
Lane 4: C2C12 cell lysate (negative) (20 µg/Lane)
Lane 5: Mouse colon tissue lysate (40 µg/Lane)
Lane 6: Mouse spleen tissue lysate (40 µg/Lane)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 129 kDa
Observed band size: 140 kDa

Exposure time: Lane 1-2: 59 seconds; Lane 3-5: 25 seconds; Lane 6: 8 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723512) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Application: IHC-Fr

Species: Mouse

Site: spleen

Sample: Frozen section

Antibody concentration: 1/500

Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue with Rabbit anti-CD11c antibody (HA723512) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723512) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD11c antibody (HA723512) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723512) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD11c antibody (HA723512) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723512) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.