Cytokeratin 8 Recombinant Antibody [SU0338] - Mouse IgG1 (Chimeric)
Catalog# HA601599
Cytokeratin 8 Recombinant Antibody [SU0338] - Mouse IgG1 (Chimeric)
-
IF-Cell
-
IHC-P
-
Human
-
HA610402
不含抗保成分
-
unconjugated
Overview
Product Name
Cytokeratin 8 Recombinant Antibody [SU0338] - Mouse IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within Human Cytokeratin 8 aa 321-370 / 483.
Species Reactivity
Human
Validated Applications
IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 54 kDa
Conjugation
unconjugated
Clone Number
SU0338
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification Method
Protein A affinity purified.
Application Dilution
-
IF-Cell
-
1:2,000
-
IHC-P
-
1:1,000
Target
Function
Keratin, type II cytoskeletal 8 also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that is encoded in humans by the KRT8 gene. It is often paired with keratin 18. Antibodies to CK8 can be used to differentiate lobular carcinoma of the breast from ductal carcinoma of the breast. In normal tissue, it reacts mainly with secretory epithelia, but not with squamous epithelium, such as that found in the skin, cervix, and esophagus. However, it also reacts with a range of malignant cells, including those derived from secretory epithelia, but also some squamous carcinomata, such as spindle cell carcinoma. It is considered useful in identifying microscopic metastases of breast carcinoma in lymph nodes, and in distinguishing Paget's disease from malignant melanoma. It also reacts with neuroendocrine tumors. Keratin 8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.
Background References
1. Ruiz A et al. Effect of hydroxychloroquine and characterization of autophagy in a mouse model of endometriosis. Cell Death Dis 7:e2059 (2016).
2. Xiao, L. et al. Three-dimensional epithelial and mesenchymal cell co-cultures form early tooth epithelium invagination-like structures: expression patterns of relevant molecules. J. Cell. Biochem. 113: 1875-1885(2012).
Subcellular Location
Nucleoplasm, Nucleus matrix, Cytoplasm.
UNIPROT
Synonyms
CARD2 antibody
Cell proliferation inducing gene 46 protein antibody
Cell proliferation inducing protein 46 antibody
CK 8 antibody
CK-8 antibody
CK8 antibody
CYK8 antibody
Cytokeratin 8 antibody
Cytokeratin-8 antibody
K2C8 antibody
ExpandImages
-
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: SK-Br-3 (Human breast cancer cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA601599, 1/2,000, overnight at 4°C.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature.
Counterstain: Beta tubulin (ET1602-4, red), 1/200, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Breast cancer
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601599, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Liver
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601599, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Colon cancer
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601599, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Cytokeratin 8 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody [SU0338] - BSA and Azide free
Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
iFluor™ 647 Conjugated Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody [SU0338]
Application: IF-Tissue,IF-Cell,FC
Reactivity: Human,Mouse
Conjugate: iFluor™ 647
Cytokeratin 8 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Cytokeratin 8 Recombinant Antibody [SU0338] - Mouse IgG1 (Chimeric) - BSA and Azide free
Application: IF-Cell,IHC-P
Reactivity: Human
Conjugate: unconjugated
Cytokeratin 8 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
iFluor™ 488 Conjugated Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody [SU0338]
Application: IF-Tissue,IF-Cell,FC
Reactivity: Human
Conjugate: iFluor™ 488
Cytokeratin 8 Mouse Monoclonal Antibody [A1-B11]
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody [SU0338]
Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
