Doublecortin Recombinant Antibody [JJ0959] - Rat IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610238
Doublecortin Recombinant Antibody [JJ0959] - Rat IgG1 (Chimeric) - BSA and Azide free
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IHC-Fr
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IHC-P
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WB
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Human
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Mouse
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Rat
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HA601398
含抗保成分
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
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unconjugated
Overview
Product Name
Doublecortin Recombinant Antibody [JJ0959] - Rat IgG1 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within Human Doublecortin aa 22-71 / 365.
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
IHC-Fr, IHC-P, WB
Target Molecular Weight
Predicted band size: 41 kDa
Positive Control
Mouse hippocampus tissue, rat hippocampus tissue, SH-SY5Y cell lysate, Mouse brain (P0) tissue lysate.
Conjugation
unconjugated
Clone Number
JJ0959
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Neuronal migration protein doublecortin, also known as doublin or lissencephalin-X is a protein that in humans is encoded by the DCX gene. Doublecortin (DCX) is a microtubule-associated protein expressed by neuronal precursor cells and immature neurons in embryonic and adult cortical structures. Neuronal precursor cells begin to express DCX while actively dividing, and their neuronal daughter cells continue to express DCX for 2–3 weeks as the cells mature into neurons. Downregulation of DCX begins after 2 weeks, and occurs at the same time that these cells begin to express NeuN, a neuronal marker.
Background References
1. Fu X et al. Doublecortin and JIP3 are neural-specific counteracting regulators of dynein-mediated retrograde trafficking. Elife. 2022 Dec
2. Li YN et al. Doublecortin-Expressing Neurons in Human Cerebral Cortex Layer II and Amygdala from Infancy to 100 Years Old. Mol Neurobiol. 2023 Jun
Subcellular Location
Cytoplasm, Cell projection, neuron projection.
Synonyms
DBCN antibody
Dbct antibody
DC antibody
DCX antibody
DCX_HUMAN antibody
Doublecortex antibody
Doublin antibody
FLJ51296 antibody
Lis X antibody
Lis-X antibody
ExpandImages
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This data was developed using HA601398, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
This data was developed using HA601398, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rat anti-Doublecortin antibody (HA601398) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601398) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601398, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rat anti-Doublecortin antibody (HA601398) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601398) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
This data was developed using HA601398, the same antibody clone in a different buffer formulation.
Western blot analysis of Doublecortin on different lysates with Rat anti-Doublecortin antibody (HA601398) at 1/5,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: A549 cell lysate (negative)
Lane 3: Mouse brain (P0) tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 41/45 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601398) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"