MAG Recombinant Antibody [PSH02-41] - Rat IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610254
MAG Recombinant Antibody [PSH02-41] - Rat IgG1 (Chimeric) - BSA and Azide free
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IHC-Fr
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IHC-P
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WB
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IF-Tissue
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Human
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Mouse
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Rat
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HA601414
含抗保成分
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
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unconjugated
Overview
Product Name
MAG Recombinant Antibody [PSH02-41] - Rat IgG1 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within human MAG aa 1-536 / 626.
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
IHC-Fr, IHC-P, WB, IF-Tissue
Target Molecular Weight
Predicted band size: 69 kDa
Positive Control
Human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate.
Conjugation
unconjugated
Clone Number
PSH02-41
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity). Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane (By similarity). Negative regulator of neurite outgrowth; in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides. In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides. In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides. Inhibits axon longitudinal growth (By similarity). Inhibits axon outgrowth by binding to RTN4R (By similarity). Preferentially binds to alpha-2,3-linked sialic acid. Binds ganglioside Gt1b (By similarity).
Background References
1. Latov N et al. Anti-MAG neuropathy: historical aspects, clinical-pathological correlations, and considerations for future therapeutical trials. Arq Neuropsiquiatr. 2024 Jun
2. Steck AJ. Anti-MAG neuropathy: From biology to clinical management. J Neuroimmunol. 2021 Dec
Subcellular Location
Cell membrane, Membrane raft.
Synonyms
GMA antibody
MAG antibody
MAG_HUMAN antibody
Myelin associated glycoprotein antibody
Myelin-associated glycoprotein antibody
S MAG antibody
S-MAG antibody
Sialic acid binding Ig like lectin 4A antibody
Sialic acid binding immunoglobulin like lectin 4A antibody
Siglec 4a antibody
ExpandImages
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This data was developed using HA601414, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
This data was developed using HA601414, the same antibody clone in a different buffer formulation.
Application: IF-tissue
Species: Mouse
Site: Cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
This data was developed using HA601414, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-MAG antibody (HA601414) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601414) at 1/500dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601414, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rat anti-MAG antibody (HA601414) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601414) at 1/500dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601414, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-MAG antibody (HA601414) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601414) at 1/500dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
This data was developed using HA601414, the same antibody clone in a different buffer formulation.
Western blot analysis of MAG on different lysates with Rat anti-MAG antibody (HA601414) at 1/2,000 dilution.
Lane 1: Mouse cerebellum tissue lysate
Lane 2: Mouse liver tissue lysate (negative)
Lane 3: Rat cerebellum tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 69 kDa
Observed band size: 100 kDa
Exposure time: Lane 1-2: 13 seconds; Lane 3: 21 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601414) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"