GFAP Recombinant Antibody [SA03-04] - Rat IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610317
GFAP Recombinant Antibody [SA03-04] - Rat IgG1 (Chimeric) - BSA and Azide free
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IHC-Fr
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IHC-P
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WB
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Mouse
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Rat
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HA601483
含抗保成分
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unconjugated
Overview
Product Name
GFAP Recombinant Antibody [SA03-04] - Rat IgG1 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within Human GFAP aa 1-50 / 432.
Species Reactivity
Mouse, Rat
Validated Applications
IHC-Fr, IHC-P, WB
Target Molecular Weight
Predicted band size: 50 kDa
Positive Control
Human brain tissue, rat brain tissue, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
SA03-04
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Glial fibrillary acidic protein (GFAP) is a protein that is encoded by the GFAP gene in humans. It is a type III intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS), including astrocytes and ependymal cells during development. GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts taken from rat kidneys, Leydig cells of the testis in both hamsters and humans, human keratinocytes, human osteocytes and chondrocytes and stellate cells of the pancreas and liver in rats. GFAP is closely related to the other three non-epithelial type III IF family members, vimentin, desmin and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength as well as the shape of cells, but its exact function remains poorly understood, despite the number of studies using it as a cell marker.
Background References
1. Zhang N et al. A self-assembly peptide nanofibrous scaffold reduces inflammatory response and promotes functional recovery in a mouse model of intracerebral hemorrhage. Nanomedicine N/A:N/A (2016).
2. Green AL et al. Preclinical antitumor efficacy of selective exportin 1 inhibitors in glioblastoma. Neuro Oncol 17:697-707 (2015).
Subcellular Location
Cytoplasm
Synonyms
wu:fb34h11 antibody
ALXDRD antibody
cb345 antibody
etID36982.3 antibody
FLJ42474 antibody
FLJ45472 antibody
GFAP antibody
GFAP_HUMAN antibody
gfapl antibody
Glial fibrillary acidic protein antibody
ExpandImages
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This data was developed using HA601483, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rat anti-GFAP antibody (HA601483) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601483) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601483, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-GFAP antibody (HA601483) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601483) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
This data was developed using HA601483, the same antibody clone in a different buffer formulation.
Western blot analysis of GFAP on different lysates with Rat anti-GFAP antibody (HA601483) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Mouse liver tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601483) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"