Total OXPHOS Antibody Cocktail Recombinant Rabbit Multiclonal Antibody [PSH15-50]
Catalog# HA723756
Total OXPHOS Antibody Cocktail Recombinant Rabbit Multiclonal Antibody [PSH15-50]
-
WB
-
IHC-P
-
Human
Overview
Product Name
Total OXPHOS Antibody Cocktail Recombinant Rabbit Multiclonal Antibody [PSH15-50]
Antibody Type
Recombinant Rabbit multiclonal
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 18/22/29/48/54 kDa
Positive Control
HEK-293 cell lysate, HeLa cell lysate, A549 cell lysate, K-562 cell lysate, MCF7 cell lysate, human breast carcinoma tissue, human liver tissue.
Conjugation
unconjugated
Clone Number
PSH15-50
RRID
Product Features
Form
Liquid
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IHC-P
-
1:200
Target
Background References
暂无
Sequence Similarity
Belongs to the ATPase alpha/beta chains family.
Tissue Specificity
Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
Post-translational Modification
The N-terminus is blocked.; Acetylated on lysine residues. BLOC1S1 is required for acetylation.
Subcellular Location
Mitochondrion inner membrane.
UNIPROT
Synonyms
ASHI antibody
ATP synthase subunit alpha antibody
Atp5a1 antibody
ATP5AL2 antibody
COII antibody
Complex I ASHI subunit antibody
COX 2 antibody
COX II antibody
COX2 antibody
COXII antibody
ExpandImages
-
Western blot analysis of Total OXPHOS Antibody Cocktail on different lysates with Rabbit anti-Total OXPHOS Antibody Cocktail antibody (HA723756) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: A549 cell lysate
Lane 4: K-562 cell lysate
Lane 5: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18/22/29/48/54 kDa
Observed band size: 18/22/29/48/54 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723756) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Total OXPHOS Antibody Cocktail antibody (HA723756) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723756) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Total OXPHOS Antibody Cocktail antibody (HA723756) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723756) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"