HRP Conjugated Human Lipocalin-2 Recombinant Rabbit Monoclonal Antibody [PSH13-68] - Detector
Overview
Product Name
HRP Conjugated Human Lipocalin-2 Recombinant Rabbit Monoclonal Antibody [PSH13-68] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Lipocalin-2 aa 21-198 (HA211028).
Species Reactivity
Human, Cynomolgus monkey
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human Lipocalin-2 protein (HA211028).
Conjugation
HRP
Clone Number
PSH13-68
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| Elisa | |
|---|---|
| human |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-67] to Human Lipocalin-2 antibody (Capture) (HA725109) and recombinant Human Lipocalin-2 protein (HA211028) as the standard. The reference range value is 140-11,100 pg/mL.
-
ELISA
-
Use at an assay dependent concentration.
Target
Function
Lipocalin-2 (LCN2), also known as oncogene 24p3 or neutrophil gelatinase-associated lipocalin (NGAL), is a protein that in humans is encoded by the LCN2 gene. NGAL is involved in innate immunity by sequestering iron and preventing its use by bacteria, thus limiting their growth. It is expressed in neutrophils and in low levels in the kidney, prostate, and epithelia of the respiratory and alimentary tracts. NGAL is used as a biomarker of kidney injury.
Background References
1. Gupta U et al. Increased LCN2 (lipocalin 2) in the RPE decreases autophagy and activates inflammasome-ferroptosis processes in a mouse model of dry AMD. Autophagy. 2023 Jan
2. Zhao RY et al. Role of lipocalin 2 in stroke. Neurobiol Dis. 2023 Apr
Subcellular Location
Secreted, Cytoplasmic granule lumen, Cytoplasmic vesicle lumen.
UNIPROT
Synonyms
24p3 antibody
25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
Alpha 2 microglobulin related protein antibody
HGNC:6526 antibody
HNL antibody
Lcn 2 antibody
Lcn2 antibody
Lipocalin-2 antibody
Migration stimulating factor inhibitor antibody
ExpandImages
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![Sandwich ELISA analysis of Human Lipocalin-2 matched pair antibodies<br /><br />Capture: <a href="/products/HA725109" style="font-weight: bold;text-decoration: underline;">HA725109</a>, Human Lipocalin-2 Rabbit mAb [PSH13-67]<br />Detector: <a href="/products/HA725110" style="font-weight: bold;text-decoration: underline;">HA725110</a>, Human Lipocalin-2 Rabbit mAb [PSH13-68]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725109" style="font-weight: bold;text-decoration: underline;">HA725109</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Lipocalin-2 protein (<a href="/products/HA211028" style="font-weight: bold;text-decoration: underline;">HA211028</a>) starting from 11,100 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA725110" style="font-weight: bold;text-decoration: underline;">HA725110</a>, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725111H_1.jpg?v=20260604174803)
Sandwich ELISA analysis of Human Lipocalin-2 matched pair antibodies
Capture: HA725109, Human Lipocalin-2 Rabbit mAb [PSH13-67]
Detector: HA725110, Human Lipocalin-2 Rabbit mAb [PSH13-68]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725109) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Lipocalin-2 protein (HA211028) starting from 11,100 pg/ml to 0 pg/ml and detect antibody (HA725110, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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