Myelin Basic Protein Recombinant Antibody [JF0943] - Chicken IgY (Chimeric) - BSA and Azide free
Catalog# HA610392
Myelin Basic Protein Recombinant Antibody [JF0943] - Chicken IgY (Chimeric) - BSA and Azide free
-
IHC-Fr
-
IF-Tissue
-
Human
-
Mouse
-
Rat
-
HA601580
含抗保成分
-
unconjugated
Overview
Product Name
Myelin Basic Protein Recombinant Antibody [JF0943] - Chicken IgY (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within Human Myelin Basic Protein aa 121-304 / 304.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IHC-Fr, IF-Tissue
Target Molecular Weight
Predicted band size: 33 kDa
Positive Control
Human brain tissue, mouse brain tissue, rat brain tissue,
Conjugation
unconjugated
Clone Number
JF0943
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Myelin basic protein (MBP) is the major extrinsic membrane protein of central nervous system myelin. MBP phosphorylation at Threonine 125 is a complex regulatory process that modulates the contribution of MBP to the stability of the myelin sheath. Mitogen-activated protein kinases modulate MBP phosphorylation during myelinogenesis and in the demyelinating disease multiple sclerosis. MBP phosphorylation is regulated by high-frequency stimulation but not low-frequency stimulation of the alveus, the myelinated output fibers of the hippocampus. It is proposed that during periods of increased neuronal activity, calcium activates axonal nitric oxide synthase, which generates the intercellular messengers nitric oxide and superoxide and regulates the phosphorylation state of MBP by MAPK.
Background References
1. Martinsen V et al. Multiple sclerosis and myelin basic protein: insights into protein disorder and disease. Amino Acids. 2022 Jan
2. Bagheri H et al. Myelin basic protein mRNA levels affect myelin sheath dimensions, architecture, plasticity, and density of resident glial cells. Glia. 2024 Oct
Subcellular Location
Myelin membrane, Nucleus.
Synonyms
GDB antibody
Golli MBP antibody
Golli MBP
myelin basic protein antibody
Hemopoietic MBP antibody
HMBPR antibody
HUGO antibody
MBP antibody
MBP_CAVPO antibody
MBP_HUMAN antibody
ExpandImages
-
This data was developed using HA601580, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: Cerebral cortex
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× PBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: HA601580, 1/200, overnight at 4℃.
Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature. -
This data was developed using HA601580, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: Hippocampus
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× PBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: HA601580, 1/200, overnight at 4℃.
Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature. -
This data was developed using HA601580, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IF-tissue)
Species: Human
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601580, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature. -
This data was developed using HA601580, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Cerebral cortex
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601580, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature. -
This data was developed using HA601580, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IF-tissue)
Species: Rat
Tissue: Cerebral cortex
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601580, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Myelin Basic Protein Recombinant Antibody [JF0943] - Chicken IgY (Chimeric)
Application: IHC-Fr,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Myelin Basic Protein Recombinant Rabbit Monoclonal Antibody [JF0943] - BSA and Azide free
Application: WB,IHC-P,IF-Tissue,IHC-Fr
Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
Conjugate: unconjugated
