HRP Conjugated Anti-mouse IgG for IP Nano-secondary antibody
Catalog# NBI02H
HRP Conjugated Anti-mouse IgG for IP Nano-secondary antibody
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WB
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IP
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Mouse
Overview
Product Name
HRP Conjugated Anti-mouse IgG for IP Nano-secondary antibody
Antibody Type
Alpaca Monoclonal Antibody
Immunogen
Mouse IgG
Species Reactivity
Mouse
Validated Applications
WB, IP
Conjugation
HRP
Product Features
Form
Liquid
Storage Instructions
Store at –20 ℃, protect from light.
Storage Buffer
PBS PH=7.5, 10mg/ml BSA, 100mM trehalose, 50% glycerol.
Isotype
IgG
Purification Method
Recombinant Expression and Affinity purified,Dissociation constant KD of 0.1nM.
Application Dilution
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WB
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1:1,000-1:10,000
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IP
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sample should be completely reduced/denatured before loaded onto a western blot.
Applications in Publications
Species in Publications
| Human | See 1 publications below |
Target
Function
Anti-mouse IgG Nano-secondary antibody for IP (HRP) is based on Monovalent, recombinant single domain antibodies to mouse IgG coupled to HRP, and the Anti-mouse IgG for IP Nano-secondary antibody (HRP) detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) selectively, no reactivity with the reduced, SDS-denatured forms. When performing immunopreciptiation (IP) followed by western blotting, the denatured mouse IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Anti-mouse IgG for IP Nano-secondary antibody (HRP) detects the non-reduced form of mouse IgG, no reactivity with the reduced, SDS-denatured forms. When a protien was immunoprecipitated by an antibody derived from mouse, you can still use primary antibody derived from mouse to detect this protien. Using Anti-mouse IgG for IP Nano-secondary antibody (HRP) to detect the primary antibody, only native antibody can be staining, not denatured heavy and light chains.
Background References
暂无
Images
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Immunoprecipitating IgG and DYKDDDDK Tag (FLAG)(M1403-2) in recombinant protein with falg on N-terminal. 25ng of Immunoprecipitated protein incubated with primary antibody (1/1000) for over night at 4℃. For western blotting a IP Detection Reagent (NBI02H) (1/1000) was used to confirm successful immunoprecipation.
Lane1/2: Immunoprecipitating IgG in recombinant protein with flag on N-terminal
Lane3: Immunoprecipitating DYKDDDDK Tag (FLAG) (M1403-2) in recombinant protein with flag on N-terminal -
PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with HA601172 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA601172 IP in HeLa cell lysate
Lane 3: Mouse IgG instead of HA601172 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 24 seconds; ECL: K1801 -
PCNA was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with HA601172 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: NIH/3T3 cell lysate (input)
Lane 2: HA601172 IP in NIH/3T3 cell lysate
Lane 3: Mouse IgG instead of HA601172 in NIH/3T3 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 5 seconds; ECL: K1802
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Adipocytes-induced ANGPTL4/KLF4 axis drives glycolysis and metastasis in triple-negative breast cancer
Author: Yin Dou, Fang Nana, Zhu Yaling, Bao Xiaoqing, Yang Juan, Zhang Qingyu, Wang Ruimeng, Huang Jiahui, Wu Qibing, Ma Fang, Wei Xiaohui
PMID: 40616161
Journal: Journal Of Experimental & Clinical Cancer Research
Application: WB
Reactivity: Human
Publish date: 2025 Jul
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Citation