HRP Conjugated Vinculin Recombinant Rabbit Monoclonal Antibody [JM42-43]
Catalog# HA724470H
HRP Conjugated Vinculin Recombinant Rabbit Monoclonal Antibody [JM42-43]
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WB
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Human
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Mouse
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Rat
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Green monkey
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Pig
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HRP
Overview
Product Name
HRP Conjugated Vinculin Recombinant Rabbit Monoclonal Antibody [JM42-43]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Vinculin aa 1,000-1,060.
Species Reactivity
Human, Mouse, Rat, Green monkey, Pig
Validated Applications
WB
Target Molecular Weight
Predicted band size: 124 kDa
Positive Control
HeLa (Human cervical adenocarcinoma cell) cell lysate, HEK-293 (Human embryonic kidney cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, C6 (Rat glioma cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, Pig brain tissue lysate, Mouse lung tissue lysate, Mouse spleen tissue lysate, Mouse colon tissue lysate, Rat lung tissue lysate, Rat spleen tissue lysate, Rat colon tissue lysate.
Conjugation
HRP
Clone Number
JM42-43
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
Target
Function
Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and α-actinin. In addition, vinculin, Talin and α-actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. α-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.
Background References
1. King HO et al. RAD51 Is a Selective DNA Repair Target to Radiosensitize Glioma Stem Cells. Stem Cell Reports 8:125-139 (2017).
2. Boyette LB et al. Phenotype, function, and differentiation potential of human monocyte subsets. PLoS One 12:e0176460 (2017).
Subcellular Location
Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane.
Synonyms
CMD1W antibody
CMH15 antibody
Epididymis luminal protein 114 antibody
HEL114 antibody
Metavinculin antibody
MV antibody
MVCL antibody
OTTHUMP00000019861 antibody
OTTHUMP00000019862 antibody
VCL antibody
ExpandImages
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Western blot analysis of Vinculin on different lysates with Rabbit anti-Vinculin antibody (HA724470H) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: HEK-293 (Human embryonic kidney cell) cell lysate
Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 4: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate
Lane 5: C6 (Rat glioma cell) cell lysate
Lane 6: COS-1 (African green monkey kidney fibroblast) cell lysate
Lane 7: Pig brain tissue lysate
Lysates/proteins at 5 µg/Lane.
Predicted band size: 124 kDa
Observed band size: 124 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724470H) at 1/2,000 dilution was used in K1803 at room temperature for 1 hour. -
Western blot analysis of Vinculin on different lysates with Rabbit anti-Vinculin antibody (HA724470H) at 1/2,000 dilution.
Lane 1: Mouse lung tissue lysate (10 µg/Lane)
Lane 2: Mouse spleen tissue lysate (10 µg/Lane)
Lane 3: Mouse colon tissue lysate (10 µg/Lane)
Lane 4: Rat lung tissue lysate (10 µg/Lane)
Lane 5: Rat spleen tissue lysate (10 µg/Lane)
Lane 6: Rat colon tissue lysate (10 µg/Lane)
Predicted band size: 124 kDa
Observed band size: 124, ~145 kDa
Exposure time: 5 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724470H) at 1/2,000 dilution was used in K1803 at room temperature for 1 hour. -
Western blot analysis of Vinculin on different lysates with Rabbit anti-Vinculin antibody (HA724470H) at 1/5,000 dilution.
Lane 1: Mouse lung tissue lysate (10 µg/Lane)
Lane 2: Mouse spleen tissue lysate (10 µg/Lane)
Lane 3: Mouse colon tissue lysate (10 µg/Lane)
Lane 4: Rat lung tissue lysate (10 µg/Lane)
Lane 5: Rat spleen tissue lysate (10 µg/Lane)
Lane 6: Rat colon tissue lysate (10 µg/Lane)
Predicted band size: 124 kDa
Observed band size: 124, ~145 kDa
Exposure time: 11 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724470H) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 1 hour. -
Western blot analysis of Vinculin on different lysates with Rabbit anti-Vinculin antibody (HA724470H) at 1/2,000 dilution.
Lane 1: Vero (African green monkey normal kidney cell)
Lane 2: RD (Human malignant embryonal rhabdomyosarcoma cell)
Predicted band size: 124 kDa
Observed band size: 124 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724470H) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 1 hour.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Vinculin Recombinant Rabbit Monoclonal Antibody [JM42-43] - BSA and Azide free
Application: WB,IF-Cell,IF-Tissue,IHC-P
Reactivity: Human,Mouse,Rat,Green monkey,Pig
Conjugate: unconjugated
