Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647 ×8)
Catalog# 001-102-009
Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647 ×8)
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WB
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ELISA
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ICC/IF
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Flow Cyt
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Mouse
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iFluor™ 647
Overview
Product Name
Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647 ×8)
Antibody Type
Alpaca Nanobody
Immunogen
Recombinant mouse IgG
Product Specificity
Mouse IgG(Fcγ fragment specific)
Species Reactivity
Mouse
Validated Applications
WB, ELISA, ICC/IF, Flow Cyt
Conjugation
iFluor™ 647
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at –20 ℃(Avoid freeze / thaw cycles), Protect from light.
Storage Buffer
Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol
Isotype
VHH domain of alpaca IgG2b/2c
Purification Method
Recombinant Expression and Affinity purified
Application Dilution
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Flow Cyt
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1:200-1:2,000
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ICC/IF
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1:200-1:2,000
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ELISA
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1:10,000-1:50,000
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WB
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1:10,000-1:50,000
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Super-resolution microscopy
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Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Target
Function
Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647 ×8) is designed for detecting mouse IgG Fcγ fragment(including mouse IgG1, IgG2a, IgG2b, IgG3) specifically, and Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647 ×8) is useful for super-resolution microscopy. Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647 ×8) is based on recombinant single domain antibody to mouse IgG Fc coupled to iFluor647. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647 ×8) reacts with the Fc fragment of mouse IgG heavy chain but not with the Fab portion of mouse IgG.
Background References
暂无
Images
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IF experiment of Hela using Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647 ×8)
Hela cells were stained with Anti-Tubulin Mouse IgG antibody (1:200) and Anti-Mouse IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647 ×8) was used as the secondary antibody(1:1000).(Left:DAPI, Right:647)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"