UBE2C Recombinant Rabbit Monoclonal Antibody [JE38-67]
Catalog# HA722454
UBE2C Recombinant Rabbit Monoclonal Antibody [JE38-67]
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WB
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Human
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unconjugated
Overview
Product Name
UBE2C Recombinant Rabbit Monoclonal Antibody [JE38-67]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human UBE2C aa 80-179 / 179.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 20 kDa
Positive Control
HeLa cell lysate, HEK-293 cell lysate, THP-1 cell lysate.
Conjugation
unconjugated
Clone Number
JE38-67
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Component of the APC/C complex, composed of at least 14 distinct subunits that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa. Within this complex, directly interacts with ANAPC2.
Background References
1. Rape M., Kirschner M.W. Autonomous regulation of the anaphase-promoting complex couples mitosis to S-phase entry. Nature 432:588-595 (2004)
2. Jin L., Williamson A., Banerjee S., Philipp I., Rape M. Mechanism of ubiquitin-chain formation by the human anaphase-promoting complex. Cell 133:653-665 (2008)
Subcellular Location
Cytosol. Nucleoplasm.Nucleus.
UNIPROT
Synonyms
Cyclin selective ubiquitin carrier protein antibody
dJ447F3.2 antibody
Mitotic specific ubiquitin conjugating enzyme antibody
UB E2C antibody
UBCH 10 antibody
UbcH10 antibody
UBE 2C antibody
Ube2c antibody
UBE2C_HUMAN antibody
Ubiquitin carrier protein C antibody
ExpandImages
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Western blot analysis of UBE2C on different lysates with Rabbit anti-UBE2C antibody (HA722454) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: THP-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722454) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"