Arc / Arg3.1 Recombinant Rabbit Monoclonal Antibody [PSH10-67]
Catalog# HA723231
Arc / Arg3.1 Recombinant Rabbit Monoclonal Antibody [PSH10-67]
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WB
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IHC-P
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IHC-Fr
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Mouse
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Rat
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HA751369
不含抗保成分
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
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unconjugated
Overview
Product Name
Arc / Arg3.1 Recombinant Rabbit Monoclonal Antibody [PSH10-67]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse Arc aa 1-396.
Species Reactivity
Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IHC-Fr
Molecular Weight
Predicted band size: 45 kDa
Positive Control
Mouse brain tissue, rat brain tissue, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH10-67
Reactivity Data
| WB | IHC-P | IHC-Fr | |
|---|---|---|---|
| Mouse |
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| Rat |
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|
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| Cynomolgus Monkey |
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|
|
| Pig |
|
|
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:200
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IHC-Fr
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1:500
Target
Function
Activity-regulated cytoskeleton-associated protein is a plasticity protein that in humans is encoded by the ARC gene. The gene is believed to derive from a retrotransposon. The protein is found in the neurons of tetrapods and other animals where it can form virus-like capsids that transport RNA between neurons. ARC mRNA is localized to activated synaptic sites in an NMDA receptor-dependent manner, where the newly translated protein is believed to play a critical role in learning and memory-related molecular processes. Arc protein is widely considered to be important in neurobiology because of its activity regulation, localization, and utility as a marker for plastic changes in the brain. Dysfunction in the production of Arc protein has been implicated as an important factor in understanding various neurological conditions, including amnesia, Alzheimer's disease, Autism spectrum disorders, and Fragile X syndrome.
Background References
1. Zhang H et al. Arc/Arg3.1 function in long-term synaptic plasticity: Emerging mechanisms and unresolved issues. Eur J Neurosci. 2021 Oct
2. Hedde PN et al. Membrane Remodeling by Arc/Arg3.1. Front Mol Biosci. 2021 Mar
Subcellular Location
Extracellular vesicle membrane.
Synonyms
activity regulated cytoskeleton associated protein antibody
activity regulated gene 3.1 protein homolog antibody
Activity-regulated cytoskeleton-associated protein antibody
Activity-regulated gene 3.1 protein homolog antibody
Arc antibody
ARC/ARG3.1 antibody
ARC_HUMAN antibody
Arg 3.1 antibody
Arg3.1 antibody
Images
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Application: IHC-Fr
Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Arc / Arg3.1 antibody (HA723231) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723231) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Arc / Arg3.1 antibody (HA723231) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723231) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Western blot analysis of Arc / Arg3.1 on different lysates with Rabbit anti-Arc / Arg3.1 antibody (HA723231) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse kidney tissue lysate (low expression)
Lane 3: Rat brain tissue lysate
Lane 4: Rat kidney tissue lysate (low expression)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 50 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723231) at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"