ALDH2 Rabbit Polyclonal Antibody
Catalog# R1407-3
ALDH2 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Human
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Mouse
-
Rat
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unconjugated
Overview
Product Name
ALDH2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human ALDH2 aa 18-80.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 56 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, SK-Br-3 cell lysate, THP-1 cell lysate, human liver tissue lysate, rat lung tissue lysate, Hela, HepG2.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
-
1:1,000
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IF-Cell
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1:200
Target
Function
Aldehyde dehydrogenase, mitochondrial is an enzyme that in humans is encoded by the ALDH2 gene located on chromosome 12. This protein belongs to the aldehyde dehydrogenase family of enzymes. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Mitochondrial aldehyde dehydrogenase belongs to the aldehyde dehydrogenase family of enzymes that catalyze the chemical transformation from acetaldehyde to acetic acid. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Additionally, ALDH2 functions as a protector against oxidative stress.
Background References
1. "Mitochondrial aldehyde dehydrogenase polymorphism in Asian and American Indian populations: detection of new ALDH2 alleles." Novoradovsky A., Tsai S.J., Goldfarb L., Peterson R., Long J.C., Goldman D. Alcohol. Clin. Exp. Res. 19:1105-1110(1995)
2. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome." Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H. J. Proteomics 96:253-262(2014)
Sequence Similarity
Belongs to the aldehyde dehydrogenase family.
Subcellular Location
Mitochondrion matrix.
Synonyms
Acetaldehyde dehydrogenase 2 antibody
Aldehyde dehydrogenase 2 family (mitochondrial) antibody
Aldehyde dehydrogenase 2 family antibody
Aldehyde dehydrogenase mitochondrial antibody
Aldehyde dehydrogenase, mitochondrial antibody
ALDH 2 antibody
ALDH class 2 antibody
ALDH E2 antibody
ALDH-E2 antibody
Aldh2 antibody
ExpandImages
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Western blot analysis of ALDH2 on different lysates with Rabbit anti-ALDH2 antibody (R1407-3) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: HepG2 cell lysate (15 µg/Lane)
Lane 3: SK-Br-3 cell lysate (15 µg/Lane)
Lane 4: THP-1 cell lysate (15 µg/Lane)
Lane 5: Human liver tissue lysate (30 µg/Lane)
Lane 6: Rat lung tissue lysate (30 µg/Lane)
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1407-3) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
ICC staining ALDH2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
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ICC staining ALDH2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
ALDH2 Recombinant Mouse Monoclonal Antibody [7-D8-R]
Application: WB,IF-Cell,IHC-P
Reactivity: Human,Rat
Conjugate: unconjugated
ALDH2 Recombinant Mouse Monoclonal Antibody [E4-D10-R]
Application: WB,IHC-P,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Products with the same target and pathway
ALDH2 Mouse Monoclonal Antibody [E4-D10]
Application: WB,IF-Cell,IF-Tissue,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
