CD11b Mouse Monoclonal Antibody [A2-B5-D2]
Catalog# EM1902-35
CD11b Mouse Monoclonal Antibody [A2-B5-D2]
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WB
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Human
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Mouse
Overview
Product Name
CD11b Mouse Monoclonal Antibody [A2-B5-D2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within mouse CD11b aa 1-300.
Species Reactivity
Human, Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 127 kDa
Positive Control
RAW264.7 cell lysates, Mouse spleen tissue lysates, A549.
Conjugation
unconjugated
Clone Number
A2-B5-D2
RRID
Product Features
Form
Liquid
Concentration
2ug/ul
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgM
Purification Method
Protein A/G affinity purified.
Application Dilution
-
WB
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1:500
Target
Function
Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (By similarity). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (By similarity). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development .
Background References
1. Coxon A.et.al.A novel role for the beta 2 integrin CD11b/CD18 in neutrophil apoptosis: a homeostatic mechanism in inflammation.Immunity 5:653-666(1996) .
Subcellular Location
Cell membrane, Membrane.
Synonyms
antigen CD11b (p170) antibody
Antigen CD11b p170 antibody
CD11 antigen like family member B antibody
CD11 antigen-like family member B antibody
CD11b antibody
CD11b/CD18 antibody
CD49d antibody
Cell surface glycoprotein MAC-1 subunit alpha antibody
Complement component 3 receptor 3 subunit antibody
Complement Component Receptor 3 Alpha antibody
ExpandImages
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Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (EM1902-35) at 1/500 dilution.
Lane 1: RAW264.7 cell lysate (RIPA lysis)
Lane 2: RAW264.7 cell lysate (hot lysis)
Lane 3: RAW264.7 cell lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 170 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-35) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CD11b on Mouse spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-35, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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