COL1A1 Recombinant Rabbit Monoclonal Antibody [ST58-04] - BSA and Azide free
Catalog# HA750188
COL1A1 Recombinant Rabbit Monoclonal Antibody [ST58-04] - BSA and Azide free
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WB
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IHC-P
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Human
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Cow
Predicted species support after-sales service
Overview
Product Name
COL1A1 Recombinant Rabbit Monoclonal Antibody [ST58-04] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human COL1A1 aa 1191-1238 / 1464.
Species Reactivity
Human (Predicted: Cow)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 139 kDa
Positive Control
Human placenta tissue lysates, human skin tissue lysates, human kidney tissue, human colon carcinoma tissue.
Conjugation
unconjugated
Clone Number
ST58-04
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IHC-P
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1:1,000
Target
Function
The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.
Background References
1. Dang PN et al. Controlled Dual Growth Factor Delivery From Microparticles Incorporated Within Human Bone Marrow-Derived Mesenchymal Stem Cell Aggregates for Enhanced Bone Tissue Engineering via Endochondral Ossification. Stem Cells Transl Med 5:206-17 (2016).
2. Huang W et al. Astragalus and Paeoniae radix rubra extract inhibits liver fibrosis by modulating the transforming growth factor- /Smad pathway in rats. Mol Med Rep 11:805-14 (2015).
Sequence Similarity
Belongs to the fibrillar collagen family.
Tissue Specificity
Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Post-translational Modification
Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.; Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline.; Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains.; O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide.
Subcellular Location
Secreted.
Synonyms
Alpha 1 type I collagen antibody
alpha1(I) procollagen antibody
CO1A1_HUMAN antibody
COL1A1 antibody
collagen alpha 1 chain type I antibody
Collagen alpha-1(I) chain antibody
collagen alpha-1(I) chain preproprotein antibody
Collagen I alpha 1 polypeptide antibody
collagen of skin, tendon and bone, alpha-1 chain antibody
Collagen type I alpha 1 antibody
ExpandImages
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Western blot analysis of COL1A1 on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750188, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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Western blot analysis of COL1A1 on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750188, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-COL1A1 antibody (HA750188) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750188) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-COL1A1 antibody (HA750188) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750188) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
COL1A1 Recombinant Rabbit Monoclonal Antibody [PSH06-20] - BSA and Azide free
Application: WB,IHC-P,IF-Tissue,IF-Cell,FC,IP,IHC-Fr
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
COL1A1 Recombinant Rabbit Monoclonal Antibody [ST58-04]
Application: WB,IHC-P
Reactivity: Human,Cow
Conjugate: unconjugated
