Choline Acetyltransferase Recombinant Rabbit Monoclonal Antibody [PSH15-05]
Catalog# HA723705
Choline Acetyltransferase Recombinant Rabbit Monoclonal Antibody [PSH15-05]
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WB
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IHC-Fr
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Human
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Mouse
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Rat
Overview
Product Name
Choline Acetyltransferase Recombinant Rabbit Monoclonal Antibody [PSH15-05]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse Choline Acetyltransferase aa 1-641.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-Fr
Molecular Weight
Predicted band size: 83 kDa
Positive Control
SH-SY5Y cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate.
Conjugation
unconjugated
Clone Number
PSH15-05
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:5,000
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IHC-Fr
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1:200-1:500
Target
Function
Choline acetyltransferase (also designated choactase, choline O-acetyltransferase) synthesizes acetylcholine in cholinergic neurons. Multiple choactase mRNAs with different 5'-noncoding regions are expressed as R-, N1, N2-, S- and M-types. N1-, N2- and R-type mRNAs produce a single short enzyme, while M-type mRNA produces both long and short enzymes. The long enzyme is targeted to the nuclei of cells, whereas the short protein is found in cytoplasm. A novel NFkB binding site is located within the nerve growth factor-responsive enhancer element that is recognized by the NFkB protein p49, but not p65 or p50. Decreased choactase expression and increased NFkB activity are associated with aging and Alzheimer's disease, indicating that p49 is a negative regulator of choactase expression and suggesting a possible mechanism for aging-associated declines in cholinergic function. Phosphorylation of choactase has been shown to enhance choactase catalytic activity. Specifically, Serine 440 is found to be the phosphorylation site in a recombinant human short choactase by protein kinase C and is involved in regulation of the enzyme catalytic activity and binding to subcellular membranes.
Background References
1. Gabalski AH et al. Circulating extracellular choline acetyltransferase regulates inflammation. J Intern Med. 2024 Mar
2. Liu J et al. Choline acetyltransferase and vesicular acetylcholine transporter are required for metamorphosis, reproduction, and insecticide susceptibility in Tribolium castaneum. Gene. 2022 Oct
Subcellular Location
Cytosol, nucleus, cytoplasm, neuron projection, presynapse.
Synonyms
Acetyl CoA choline O acetyltransferase antibody
Acetyl CoA:choline O acetyltransferase antibody
ChAT antibody
CHOACTase antibody
Choline acetylase antibody
choline acetyltransferase antibody
Choline O acetyltransferase antibody
Choline O-acetyltransferase antibody
CLAT_HUMAN antibody
CMS1A antibody
ExpandImages
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Western blot analysis of Choline Acetyltransferase on different lysates with Rabbit anti-Choline Acetyltransferase antibody (HA723705) at 1/5,000 dilution.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: Neuro-2a cell lysate (negative) (20 µg/Lane)
Lane 3: Mouse brain tissue lysate (40 µg/Lane)
Lane 4: Mouse cerebellum tissue lysate (40 µg/Lane)
Lane 5: Rat brain tissue lysate (40 µg/Lane)
Lane 6: Rat cerebellum tissue lysate (40 µg/Lane)
Predicted band size: 83 kDa
Observed band size: 50/75 kDa
Exposure time: 3 minutess; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723705) at 1/5,000 dilution was used in primary antibody dilution (K1803) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IHC-Fr
Species: Mouse
Site: brain
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: brain
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Choline Acetyltransferase Recombinant Rabbit Monoclonal Antibody [JA67-11]
Application: WB,IP,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
