HRH1 Rabbit Polyclonal Antibody
Catalog# ER1910-82
HRH1 Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
HRH1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
KLH conjugated synthetic peptide derived from human HRH1 141-240/487
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Tissue
Molecular Weight
54 KDa
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage Buffer
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-2000
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IHC-P
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1:100-500
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IF-tissue
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1:100-500
Target
Function
In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system.
Background References
暂无
Subcellular Location
Cell membrane; Multi-pass membrane protein.
UNIPROT
Synonyms
BPHS
mouse
homolog of antibody
H1 R antibody
H1R antibody
HH1R antibody
HisH1 antibody
Histamine H(1) receptor antibody
Histamine H1 receptor antibody
Histamine receptor H1 antibody
ExpandImages
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Sample:
Lane 1: U87MG (Human) Cell Lysate at 30 ug
Lane 2: U251 (Human) Cell Lysate at 30 ug
Lane 3: Huvec (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Primary: Anti-HRH1 (ER1910-82) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD -
Sample:
LOVO(Human) Cell Lysate at 30 ug
Primary: Anti-HRH1 (ER1910-82) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD -
Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HRH1) Polyclonal Antibody, Unconjugated (ER1910-82) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Kukoamine A attenuates allergic rhinitis via H1R antagonism: Dual suppression of inflammatory signaling and epithelial barrier disruption
Journal: Biochemical Pharmacology
DOI: 10.1016/j.bcp.2026.117792
IF: 5.6
Application: WB
Reactivity: Human
Publish date: 2026 Feb
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Investigation of the Binding Characteristics of Agonists and Various Antagonists Targeting Histamine 1 Receptor
Journal: Pharmaceutical Research
DOI: 10.1007/s11095-025-03899-z
IF: 4.3
Application: WB
Reactivity: Human
Publish date: 2025 Aug