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HRP Conjugated beta Actin Recombinant Rabbit Monoclonal Antibody [JF53-10]

Specification

Quota

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Catalog# ET1702-67

HRP Conjugated beta Actin Recombinant Rabbit Monoclonal Antibody [JF53-10]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Predicted reactivity

  • Monkey

  • Hamster

  • Arabidopsis thaliana

  • Rice

Predicted species support after-sales service
Conjugation

  • HRP

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

HRP Conjugated beta Actin Recombinant Rabbit Monoclonal Antibody [JF53-10]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within N-terminal residues of β-Actin.

Species Reactivity

Human, Mouse, Rat (Predicted: Monkey, Hamster, Arabidopsis thaliana, Rice)

Predicted species support after-sales service

Validated Applications

WB

Molecular Weight

Predicted band size: 42 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, A431 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Mouse spleen tissue lysate, Rat testis tissue lysate, Rat spleen tissue lysate.

Conjugation

HRP

Clone Number

JF53-10

RRID

AB_2890197

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:20,000-1:50,000

Target

Function

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

Background References

1. Wang H et al. Role of the orexin (hypocretin) system in contextual fear conditioning in rats. Behav Brain Res 316:47-53 (2017).

2. Gruber R et al. YAP1 and TAZ Control Pancreatic Cancer Initiation in Mice by Direct Up-regulation of JAK-STAT3 Signaling. Gastroenterology 151:526-39 (2016).

Sequence Similarity

Belongs to the actin family.

Post-translational Modification

ISGylated.; Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.; Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.; Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity).; [Actin, cytoplasmic 1, N-terminally processed]: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected.; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P60709 Human

SwissProt: P60710 Mouse

SwissProt: P60711 Rat

Synonyms

A26C1A antibody

A26C1B antibody

ACTB antibody

ACTB_HUMAN antibody

Actin beta antibody

Actin cytoplasmic 1 antibody

Actin, cytoplasmic 1, N-terminally processed antibody

Actx antibody

b actin antibody

Beta cytoskeletal actin antibody

Expand

Images

  • Western blot analysis of beta Actin on different lysates with Rabbit anti-beta Actin antibody (<a href="/products/ET1702-67" style="font-weight: bold;text-decoration: underline;">ET1702-67</a>) at 1/20,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: MCF7 cell lysate<br />Lane 4: A431 cell lysate<br />Lane 5: Jurkat cell lysate<br />Lane 6: HEK-293 cell lysate<br />Lane 7: RAW264.7 cell lysate<br />Lane 8: C2C12 cell lysate<br />Lane 9: PC-12 cell lysate<br />Lane 10: Mouse testis tissue lysate<br />Lane 11: Mouse spleen tissue lysate<br />Lane 12: Rat testis tissue lysate<br />Lane 13: Rat spleen tissue lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-67" style="font-weight: bold;text-decoration: underline;">ET1702-67</a>) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

    Western blot analysis of beta Actin on different lysates with Rabbit anti-beta Actin antibody (ET1702-67) at 1/20,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: MCF7 cell lysate
    Lane 4: A431 cell lysate
    Lane 5: Jurkat cell lysate
    Lane 6: HEK-293 cell lysate
    Lane 7: RAW264.7 cell lysate
    Lane 8: C2C12 cell lysate
    Lane 9: PC-12 cell lysate
    Lane 10: Mouse testis tissue lysate
    Lane 11: Mouse spleen tissue lysate
    Lane 12: Rat testis tissue lysate
    Lane 13: Rat spleen tissue lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-67) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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