GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody [JE60-09]
Catalog# HA721338
GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody [JE60-09]
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
Overview
Product Name
GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody [JE60-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human GABA B Receptor 1 aa 35-134 / 961.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 108 kDa
Positive Control
K-562 cell lysate, Jurkat cell lysate, HeLa cell lysate, SHG-44 cell lysate, mouse brain tissue.
Conjugation
unconjugated
Clone Number
JE60-09
RRID
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
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IF-Tissue
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1:100
Target
Function
Gamma-aminobutyric acid B receptor, 1 (GABAB1), is a G-protein coupled receptor subunit encoded by the GABBR1 gene. GABAB1 is a receptor for Gamma-aminobutyric acid. Upon binding, GABAB1 will produce a slow and prolonged inhibitory effect. GABAB1 is one part of a heterodimer, which is the GABAB receptor, consisting of it and the related GABAB2 protein. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates 4 transcript variants. GABBR1 has been shown to interact with ATF4 and GABBR2.
Background References
1. Shao L et al. The neurotransmitter receptor Gabbr1 regulates proliferation and function of hematopoietic stem and progenitor cells. Blood. 2021 Feb
2. Cediel ML et al. GABBR1 monoallelic de novo variants linked to neurodevelopmental delay and epilepsy. Am J Hum Genet. 2022 Oct
Subcellular Location
Cell membrane, Postsynaptic cell membrane, Cell projection, dendrite; Secreted.
Synonyms
dJ271M21.1.1 antibody
dJ271M21.1.2 antibody
FLJ92613 antibody
GABA-B receptor 1 antibody
GABA-B-R1 antibody
GABA-BR1 antibody
GABAB R1 antibody
GABAB subunit 1c antibody
GABABR1 antibody
GABBR1 3 antibody
ExpandImages
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Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody (HA721338) at 1/1,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HeLa cell lysate
Lane 4: SHG-44 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 108 kDa
Observed band size: 108 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721338) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GABA B Receptor 1 antibody (HA721338) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721338) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling GABA B Receptor 1 with Rabbit anti-GABA B Receptor 1 antibody (HA721338) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721338, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody [PSH03-87]
Application: WB,IF-Tissue,IHC-P
Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
Conjugate: unconjugated
