GFAP Recombinant Mouse Monoclonal Antibody [PSH10-24] - BSA and Azide free

This data was developed using HA601380, the same antibody clone in a different buffer formulation.
Application: IHC-Fr

Species: Mouse

Site: Hippocampus

Sample: Frozen section

Antibody concentration: 1/500

Antigen retrieval: Not required

This data was developed using HA601380, the same antibody clone in a different buffer formulation.
Application: IHC-Fr

Species: Rat

Site: Cerebral cortex

Sample: Frozen section

Antibody concentration: 1/500

Antigen retrieval: Not required

☑ Relative expression (RE)

This data was developed using HA601380, the same antibody clone in a different buffer formulation.
Western blot analysis of GFAP on different lysates with Mouse anti-GFAP antibody (HA601380) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (negative)
Lane 2: Human brain tissue lysate
Lane 3: Mouse brain tissue lysate
Lane 4: Rat brain tissue lysate
Lane 5: Rat cerebellum tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 50 kDa
Observed band size: 50 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601380) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

This data was developed using HA601380, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-GFAP antibody (HA601380) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601380) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

This data was developed using HA601380, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-GFAP antibody (HA601380) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601380) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

This data was developed using HA601380, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-GFAP antibody (HA601380) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601380) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

This data was developed using HA601380, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-GFAP antibody (HA601380) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601380) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

This data was developed using HA601380, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-GFAP antibody (HA601380) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601380) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

This data was developed using HA601380, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-GFAP antibody (HA601380) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601380) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

This data was developed using HA601380, the same antibody clone in a different buffer formulation.
Application: IF-Tissue

Species: Rat

Site: brain

Sample: Paraffin-embedded section

Antibody concentration: 1/500