iFluor™ 488 Conjugated Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody [SA0377]
Catalog# HA720154F
iFluor™ 488 Conjugated Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody [SA0377]
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IF-Cell
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IF-Tissue
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Human
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Mouse
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Rat
Predicted species support after-sales service
Overview
Product Name
iFluor™ 488 Conjugated Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody [SA0377]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human GLUT1 aa 443-492 / 492.
Species Reactivity
Human, Mouse (Predicted: Rat)
Predicted species support after-sales service
Validated Applications
IF-Cell, IF-Tissue
Molecular Weight
Predicted band size: 54 kDa
Positive Control
MCF7, human placenta tissue, MDA-MB-468.
Conjugation
iFluor™ 488
Clone Number
SA0377
RRID
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Cell
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1:100
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IF-Tissue
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1:50
Target
Function
Glucose is fundamental to the metabolism of mammalian cells. Its passage across cell membranes is mediated by a family of transporters termed glucose transporters or Gluts. In adipose and muscle tissue, insulin stimulates a rapid and dramatic increase in glucose uptake, which is largely due to the redistribution of the insulin-inducible glucose transporter, Glut4. In response to insulin, Glut4 is quickly shuttled from an intracellular storage site to the plasma membrane, where it binds glucose. In contrast, the ubiquitously expressed glucose transporter Glut1 is constitutively targeted to the plasma membrane, and shows a much less dramatic translocation in response to insulin. Glut1 and Glut4 are twelve-pass transmembrane proteins (12TM) whose carboxy-termini may dictate their cellular localization. Aberrant Glut4 expression has been suggested to contribute to such maladies as obesity and diabetes. Glut4 null mice have shown that while functional Glut4 protein is not required for maintaining normal glucose levels, it is necessary for sustained growth, normal cellular glucose, fat metabolism and prolonged longevity.
Background References
1. Boyer-Di Ponio J et al. Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes. PLoS One 9:e84179 (2014).
2. Saucillo DC et al. Leptin metabolically licenses T cells for activation to link nutrition and immunity. J Immunol 192:136-44 (2014).
Subcellular Location
Cell membrane, Melanosome
Synonyms
Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
CSE antibody
DYT17 antibody
DYT18 antibody
DYT9 antibody
EIG12 antibody
erythrocyte/brain antibody
Erythrocyte/hepatoma glucose transporter antibody
facilitated glucose transporter member 1 antibody
Glucose transporter 1 antibody
ExpandImages
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Immunocytochemistry analysis of MCF7 cells labeling Glucose Transporter GLUT1 with Rabbit anti-Glucose Transporter GLUT1 antibody (HA720154F) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody (HA720154F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution. -
Immunofluorescence analysis of paraffin-embedded human placenta tissue labeling Glucose Transporter GLUT1 (HA720154F).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Glucose Transporter GLUT1 (HA720154F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. -
Immunocytochemistry analysis of MDA-MB-468 cells labeling Glucose Transporter GLUT1 with Rabbit anti-Glucose Transporter GLUT1 antibody (HA720154F) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody (HA720154F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody [SA0377]
Application: WB,IF-Cell,IF-Tissue,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
