HRP Conjugated Rabbit anti-Guinea pig IgG polyclonal Antibody
Catalog# HA1021
HRP Conjugated Rabbit anti-Guinea pig IgG polyclonal Antibody
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WB
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ELISA
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Guinea pig
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HRP
Overview
Product Name
HRP Conjugated Rabbit anti-Guinea pig IgG polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Purified native Guinea Pig IgG.
Species Reactivity
Guinea pig
Validated Applications
WB, ELISA
Target Molecular Weight
55/25 kDa
Positive Control
Guinea pig IgG.
Conjugation
HRP
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:5,000-1:40,000
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ELISA
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1:5,000-1:20,000
Target
Function
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
Background References
暂无
Images
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Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Rabbit anti-Guinea pig IgG-HRP (HA1021) was used for 1 hour at room temperature at 1:10,000 (Lane 1) and 1:5,000 (Lane 2).
Lane 1: Guinea pig (30 ng per lane).
Lane 2: Mouse IgG (30 ng per lane).
Exposure time: 1 minutes -
Immobilized guinea pig IgG protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA1021) for 1 hour at 25℃.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"