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iFluor™ 647 Conjugated Goat anti-mouse IgG polyclonal Antibody

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Catalog# HA1127

iFluor™ 647 Conjugated Goat anti-mouse IgG polyclonal Antibody

Application

  • IF-Cell

  • IF-Tissue

  • IHC-Fr

Reactivity

  • Mouse

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

iFluor™ 647 Conjugated Goat anti-mouse IgG polyclonal Antibody

Antibody Type

Goat Polyclonal Antibody

Immunogen

Mouse IgG (H+L).

Product Specificity

To minimize cross-reactivity, the iFluor™ 647 conjugated goat anti-mouse IgG (H+L) antibodies have been highly cross-adsorbed against human IgG.

Species Reactivity

Mouse

Validated Applications

IF-Cell, IF-Tissue, IHC-Fr

Conjugation

iFluor™ 647

Product Features

Form

Liquid

Concentration

2ug/ul

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • IF-Cell

  • 1:500-1:1,000

  • IF-Tissue

  • 1:500-1:1,000

  • IHC-Fr

  • 1:500-1:1,000

Target

Function

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. iFluor™ 647 dyes have fluorescence excitation and emission maxima of ~656 nm and ~670 nm respectively. iFluor™ 647 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative. iFluor™ 647 is much easier to be conjugated with RPE with much higher conjugation yield, and the resulted RPE-iFluor™ 647 tandem has better FRET efficiency. iFluor™ 647 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

Background References

暂无

Images

  • Immunocytochemistry analysis of Hela cells labeling Beta Ⅲ tubulin with Mouse anti-Beta Ⅲ tubulin antibody (<a href="/products/M0805-8" style="font-weight: bold;text-decoration: underline;">M0805-8</a>) at 1/400 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 3% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Beta Ⅲ tubulin antibody (<a href="/products/M0805-8" style="font-weight: bold;text-decoration: underline;">M0805-8</a>) at 1/400 dilution in 3% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 647, <a href="/products/HA1127" style="font-weight: bold;text-decoration: underline;">HA1127</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of Hela cells labeling Beta Ⅲ tubulin with Mouse anti-Beta Ⅲ tubulin antibody (M0805-8) at 1/400 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 3% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Beta Ⅲ tubulin antibody (M0805-8) at 1/400 dilution in 3% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling beta Ⅲ tubulin (<a href="/products/M0805-8" style="font-weight: bold;text-decoration: underline;">M0805-8</a>) and Iba1 (<a href="/products/ET1705-78" style="font-weight: bold;text-decoration: underline;">ET1705-78</a>).<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies beta Ⅲ tubulin (<a href="/products/M0805-8" style="font-weight: bold;text-decoration: underline;">M0805-8</a>, red) at 1/1,000 dilution and Iba1 (<a href="/products/ET1705-78" style="font-weight: bold;text-decoration: underline;">ET1705-78</a>, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.<br /><br />iFluor&trade; 647 conjugate-Goat anti-Mouse IgG (<a href="/products/HA1127" style="font-weight: bold;text-decoration: underline;">HA1127</a>) and iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded mouse hippocampus tissue labeling beta Ⅲ tubulin (M0805-8) and Iba1 (ET1705-78).

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies beta Ⅲ tubulin (M0805-8, red) at 1/1,000 dilution and Iba1 (ET1705-78, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.

    iFluor™ 647 conjugate-Goat anti-Mouse IgG (HA1127) and iFluor™ 488 conjugate-Goat anti-Rabbit IgG (HA1121) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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  3. IHC
  4. IF-cell
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