CD34 Recombinant Mouse Monoclonal Antibody [PDM0-12]
Catalog# HA601109
CD34 Recombinant Mouse Monoclonal Antibody [PDM0-12]
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IHC-P
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IF-Tissue
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Human
Overview
Product Name
CD34 Recombinant Mouse Monoclonal Antibody [PDM0-12]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human CD34 aa 32-385/385.
Species Reactivity
Human
Validated Applications
IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 41 kDa
Positive Control
Human liver tissue, human liver carcinoma tissue, human kidney tissue, human placenta tissue, human colon tissue.
Conjugation
unconjugated
Clone Number
PDM0-12
RRID
Product Features
Form
Liquid
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
IHC-P
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1:1,000-1:5,000
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IF-Tissue
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1:1,000
Target
Function
CD34 (also named myeloid progenitor cell antigen) is a heavily glycosylated type I transmembrane protein. CD34 is detected in myeloid blasts in myelodysplastic syndrome and acute myeloid leukaemia in most cases as well as lymphoblasts in most cases of B-acute lymphoblastic leukaemia. Mature B- and T-cell lymphomas and leukaemias are CD34 negative. The majority of vascular tumours, including haemangiosarcoma and Kaposi sarcoma are CD34 positive, the protein frequently being expressed in abluminal processes. However, only about 30% of the lymphangiomas are CD34 positive. CD34 positivity is seen in most cases of dermatofibrosarcoma protuberans, solitary fibrous tumor, lipoma (particularly spindle cell lipoma) and liposarcoma, gastrointestinal stromal tumor (strong positivity in about 80% of the cases, which are also CD117 positive), and a varying proportion of meningioma. Moderate expression is seen in cutaneous leiomyoma, while weak expression is present in uterine and soft tissue smooth muscle tumours. Some cases of myofibroblastoma have revealed CD34 positivity. Schwannoma is CD34 positive mainly in Antoni B areas. Neurofibroma and neurofibrosarcoma may also stain. About 50% of epitheloid sarcomas show strong focal or generalized staining for this antigen. Some synovial sarcomas may show focal staining. Fibrous histiocytoma is CD34 negative. CD34 is expressed only exceptionally in carcinomas (kidney, thyroid gland) and clear cell sarcoma/melanoma. In a panel, CD34 staining is useful for the classification of myeloid and lymphoid neoplasms as well as spindle cell neoplasms (particularly identification of gastrointestinal stromal tumour and haemangiosarcoma). Liver and appendix is recommended as positive and negative tissue controls for CD34. In liver the protocol must be calibrated to provide a moderate to strong predominately membranous staining reaction of endothelial cells in the portal vessels, but also of the periportal sinusoidal endothelial cells serving as “low expressors” for an optimal calibrated assay for CD34. No staining reaction must be seen in liver cells.
Background References
1. Torlakovic G, et al. CD34/QBEND10 immunostaining in the bone marrow trephine biopsy: a study of CD34-positive mononuclear cells and megakaryocytes. Arch Pathol Lab Med. 2002; 126:823-8.
2. Kisluk J, et al. Immunohistochemical diagnosis of gastrointestinal stromal tumors - an analysis of 80 cases from 2004 to 2010. Adv. Clin Exp Med. 2013; 22:33-9.
Subcellular Location
Membrane.
UNIPROT
Synonyms
CD34 antibody
CD34 antigen antibody
CD34 molecule antibody
CD34_HUMAN antibody
Cluster designation 34 antibody
cluster of differentiation 34 antibody
Hematopoietic progenitor cell antigen CD34 antibody
HPCA1 antibody
Mucosialin antibody
OTTHUMP00000034733 antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-CD34 antibody (HA601109) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601109) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Mouse anti-CD34 antibody (HA601109) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601109) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-CD34 antibody (HA601109) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601109) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-CD34 antibody (HA601109) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601109) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-CD34 antibody (HA601109) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601109) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Human
Site: kidney
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000 -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-CD34 antibody (HA601109) at 1/1,000 dilution.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. The section was incubated with HA601109 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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