CD11c Recombinant Antibody [SI19-06] - Mouse IgG1 (Chimeric)
Catalog# HA601545
CD11c Recombinant Antibody [SI19-06] - Mouse IgG1 (Chimeric)
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IF-Tissue
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IHC-P
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Human
Overview
Product Name
CD11c Recombinant Antibody [SI19-06] - Mouse IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within Human CD11c aa 1,114-1,163 / 1,163 (Cytoplasmic).
Species Reactivity
Human
Validated Applications
IF-Tissue, IHC-P
Molecular Weight
Predicted band size: 128 kDa
Positive Control
Human spleen tissue, human Hodgkin's lymphoma tissue, human tonsil tissue.
Conjugation
unconjugated
Clone Number
SI19-06
Product Features
Form
Liquid
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Tissue
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1:500
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IHC-P
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1:800
Target
Function
Integrin αX (CD11C, leukocyte surface antigen p150,95, CR4, Axb2) is a type 1 transmembrane protein that traditionally combines with β2 chain to form a leukocyte-specific integrin known as inactivated-C3b (iC3b) receptor 4 (CR4). Integrin αX/β2 shares similar properties of the αM/β2 integrin in mediating adherence of neutrophils and monocytes to stimulated endothelial cells, and in phagocytosis of complement coated particles. Abnormal expression of Integrin αX is characteristic of hairy cell leukemia (HCL) and is dependent upon activation of proto-oncogenes Ras and JunD. Proteins and DNA elements that influence transcription of Integrin αX include Sp1 and Sp1-like factors, AP-1 family, C/EBP, Oct-2 and PU.1. Integrin αX is present on monocyte derivative dendritic cells (DCs), macrophages and NK cells. Upon activation, DCs present in skin (Langerhans cells), lining of nose, lung, stomach, intestine and blood can migrate to lymphoid tissues and interact with T and B cells to initiate and shape the immune response.
Background References
1. Morandi F et al. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation. J Immunol 192:2634-42 (2014).
2. Svensson MN et al. Fms-like tyrosine kinase 3 ligand controls formation of regulatory T cells in autoimmune arthritis. PLoS One 8:e54884 (2013).
Subcellular Location
Membrane.
UNIPROT
Synonyms
95 alpha chain antibody
95 antibody
CD 11c antibody
CD11 antigen-like family member C antibody
CD11c antibody
CD11c antigen antibody
Complement component 3 receptor 4 subunit antibody
CR4 antibody
Integrin alpha X antibody
Integrin alpha X chain antibody
ExpandImages
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Application: IF-Tissue
Species: Human
Site: spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD11c antibody (HA601545) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601545) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue with Mouse anti-CD11c antibody (HA601545) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601545) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD11c antibody (HA601545) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601545) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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