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HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] - Loading control

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Catalog# HA601585H

HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] - Loading control

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

  • Pig

Conjugation

  • HRP

Overview

Product Name

HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] - Loading control

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within N-terminal human Beta-actin .

Species Reactivity

Human, Mouse, Rat, Green monkey, Pig

Validated Applications

WB

Target Molecular Weight

Predicted band size: 42 kDa

Positive Control

Mouse brain tissue lysate, Mouse liver tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate, Pig brain tissue lysate, HeLa cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, COS-1 cell lysate.

Conjugation

HRP

Clone Number

A2-F6-R

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

Target

Function

Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Beta-actin has been shown to interact with SPTBN2. In addition, RNA-binding protein Sam68 was found to interact with the mRNA encoding β-actin, which regulates the synaptic formation of the dendritic spines with its cytoskeletal components. Beta-actin has been shown to activate eNOS, thereby increasing NO production. An eight-amino acid residue (326-333) in actin has been shown to mediate the interaction between actin and eNOS. Recurrent mutations in this gene have been associated to cases of diffuse large B-cell lymphoma. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.

Background References

1. Fujiki R et al. GlcNAcylation of a histone methyltransferase in retinoic-acid-induced granulopoiesis. Nature 459:455-459 (2009).

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P60709 Human

SwissProt: P60710 Mouse

SwissProt: P60711 Rat

Synonyms

A26C1A antibody

A26C1B antibody

ACTB antibody

ACTB_HUMAN antibody

Actin beta antibody

Actin cytoplasmic 1 antibody

Actin, cytoplasmic 1, N-terminally processed antibody

Actx antibody

b actin antibody

Beta cytoskeletal actin antibody

Expand

Images

  • Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (<a href="/products/HA601585H" style="font-weight: bold;text-decoration: underline;">HA601585H</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Mouse liver tissue lysate<br />Lane 3: Rat brain tissue lysate<br />Lane 4: Rat liver tissue lysate<br />Lane 4: Pig brain tissue lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 34 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601585H" style="font-weight: bold;text-decoration: underline;">HA601585H</a>, HRP) at 1/2,000 dilution was used in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at room temperature for 1 hour.

    Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (HA601585H) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse liver tissue lysate
    Lane 3: Rat brain tissue lysate
    Lane 4: Rat liver tissue lysate
    Lane 4: Pig brain tissue lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 34 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601585H, HRP) at 1/2,000 dilution was used in primary antibody dilution buffer (K1803) at room temperature for 1 hour.

  • Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (<a href="/products/HA601585H" style="font-weight: bold;text-decoration: underline;">HA601585H</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HEK-293 cell lysate<br />Lane 3: NIH/3T3 cell lysate<br />Lane 4: RAW264.7 cell lysate<br />Lane 5: C6 cell lysate<br />Lane 6: PC-12 cell lysate<br />Lane 7: COS-1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 14 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601585H" style="font-weight: bold;text-decoration: underline;">HA601585H</a>, HRP) at 1/2,000 dilution was used in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at room temperature for 1 hour.

    Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (HA601585H) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HEK-293 cell lysate
    Lane 3: NIH/3T3 cell lysate
    Lane 4: RAW264.7 cell lysate
    Lane 5: C6 cell lysate
    Lane 6: PC-12 cell lysate
    Lane 7: COS-1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 14 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601585H, HRP) at 1/2,000 dilution was used in primary antibody dilution buffer (K1803) at room temperature for 1 hour.

  • Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (<a href="/products/HA601585H" style="font-weight: bold;text-decoration: underline;">HA601585H</a>) at 1/5,000 dilution.<br /><br />Lane 1: Mouse lung tissue lysate<br />Lane 2: Mouse spleen tissue lysate<br />Lane 3: Mouse colon tissue lysate<br />Lane 3: Rat lung tissue lysate<br />Lane 4: Rat spleen tissue lysate<br />Lane 4: Rat colon tissue lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601585H" style="font-weight: bold;text-decoration: underline;">HA601585H</a>, HRP) at 1/5,000 dilution was used in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at room temperature for 1 hour.

    Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (HA601585H) at 1/5,000 dilution.

    Lane 1: Mouse lung tissue lysate
    Lane 2: Mouse spleen tissue lysate
    Lane 3: Mouse colon tissue lysate
    Lane 3: Rat lung tissue lysate
    Lane 4: Rat spleen tissue lysate
    Lane 4: Rat colon tissue lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601585H, HRP) at 1/5,000 dilution was used in primary antibody dilution buffer (K1803) at room temperature for 1 hour.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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