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beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] - BSA and Azide free

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Catalog# HA610019

beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within N-terminal human Beta-actin .

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 42 kDa

Positive Control

HepG2 cell lysates, NIH/3T3 cell lysates, PC-12 cell lysates, MCF-7 cell lysate, THP-1 cell lysate, PMVEC cell lysate, RH-35 cell lysate, RAW264.7 cell lysate, B16F1 cell lysate, MEF cell lysate, Hela cell lysate, F9 cell lysate, Mouse lung cell lysate, L6 cell lysate.

Conjugation

unconjugated

Clone Number

A2-F6-R

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000-1:80,000

Target

Function

Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Beta-actin has been shown to interact with SPTBN2. In addition, RNA-binding protein Sam68 was found to interact with the mRNA encoding β-actin, which regulates the synaptic formation of the dendritic spines with its cytoskeletal components. Beta-actin has been shown to activate eNOS, thereby increasing NO production. An eight-amino acid residue (326-333) in actin has been shown to mediate the interaction between actin and eNOS. Recurrent mutations in this gene have been associated to cases of diffuse large B-cell lymphoma. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.

Background References

1. Fujiki R et al. GlcNAcylation of a histone methyltransferase in retinoic-acid-induced granulopoiesis. Nature 459:455-459 (2009).

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P60709 Human

SwissProt: P60710 Mouse

SwissProt: P60711 Rat

Synonyms

A26C1A antibody

A26C1B antibody

ACTB antibody

ACTB_HUMAN antibody

Actin beta antibody

Actin cytoplasmic 1 antibody

Actin, cytoplasmic 1, N-terminally processed antibody

Actx antibody

b actin antibody

Beta cytoskeletal actin antibody

Expand

Images

  • Western blot analysis of beta Actin on HepG2 cell lysates with Mouse anti-beta Actin antibody (<a href="/products/HA610019" style="font-weight: bold;text-decoration: underline;">HA610019</a>) at different dilutions.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br />Exposure time: 5 minutes;<br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610019" style="font-weight: bold;text-decoration: underline;">HA610019</a>) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of beta Actin on HepG2 cell lysates with Mouse anti-beta Actin antibody (HA610019) at different dilutions.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa
    Exposure time: 5 minutes;
    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610019) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of beta Actin on NIH/3T3 cell lysates with Mouse anti-beta Actin antibody (<a href="/products/HA610019" style="font-weight: bold;text-decoration: underline;">HA610019</a>) at different dilutions.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 5 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610019" style="font-weight: bold;text-decoration: underline;">HA610019</a>) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of beta Actin on NIH/3T3 cell lysates with Mouse anti-beta Actin antibody (HA610019) at different dilutions.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 5 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610019) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of beta Actin on PC-12 cell lysates with Mouse anti-beta Actin antibody (<a href="/products/HA610019" style="font-weight: bold;text-decoration: underline;">HA610019</a>) at different dilutions.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 1 minute;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610019" style="font-weight: bold;text-decoration: underline;">HA610019</a>) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of beta Actin on PC-12 cell lysates with Mouse anti-beta Actin antibody (HA610019) at different dilutions.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 1 minute;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610019) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (<a href="/products/HA610019" style="font-weight: bold;text-decoration: underline;">HA610019</a>) at 1/20,000 dilution.<br /><br />Lane 1: Hela (Human cervical adenocarcinoma cell) cell lysate<br />Lane 2: HepG2 (Human hepatocellular carcinoma cell) cell lysate<br />Lane 3: MCF-7 (Human breast cancer cell) cell lysate<br />Lane 4: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate<br />Lane 5: Jurkat (Human T-lymphoblastic cells) cell lysate<br />Lane 6: HEK-293 (Human embryonic kidney cell) cell lysate<br />Lane 7: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate<br />Lane 8: C2C12 (Mouse myoblast) cell lysate<br />Lane 9: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate<br />Lane 10: mouse testis tissue lysate<br />Lane 11: mouse kidney tissue lysate<br />Lane 12: mouse testis tissue lysate<br />Lane 13: mouse kidney tissue lysate<br /> <br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 20 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA610019" style="font-weight: bold;text-decoration: underline;">HA610019</a>, 1/20,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa

    Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (HA610019) at 1/20,000 dilution.

    Lane 1: Hela (Human cervical adenocarcinoma cell) cell lysate
    Lane 2: HepG2 (Human hepatocellular carcinoma cell) cell lysate
    Lane 3: MCF-7 (Human breast cancer cell) cell lysate
    Lane 4: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate
    Lane 5: Jurkat (Human T-lymphoblastic cells) cell lysate
    Lane 6: HEK-293 (Human embryonic kidney cell) cell lysate
    Lane 7: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate
    Lane 8: C2C12 (Mouse myoblast) cell lysate
    Lane 9: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
    Lane 10: mouse testis tissue lysate
    Lane 11: mouse kidney tissue lysate
    Lane 12: mouse testis tissue lysate
    Lane 13: mouse kidney tissue lysate

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA610019, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1006), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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