VGLUT1 Recombinant Mouse Monoclonal Antibody [PSH08-34] - BSA and Azide free
Catalog# HA610217
VGLUT1 Recombinant Mouse Monoclonal Antibody [PSH08-34] - BSA and Azide free
-
WB
-
IHC-P
-
Mouse
-
Rat
-
HA601371
含抗保成分
-
unconjugated
Overview
Product Name
VGLUT1 Recombinant Mouse Monoclonal Antibody [PSH08-34] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within rat VGluT1 aa 470-560.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 62 kDa
Positive Control
Mouse brain tissue lysate, Rat brain tissue lysate, mouse striatum tissue, mouse cerebellum tissue, rat striatum tissue, rat cerebellum tissue.
Conjugation
unconjugated
Clone Number
PSH08-34
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Vesicular glutamate transporter 1 (VGLUT1) is a protein that in humans is encoded by the SLC17A7 gene. The protein encoded by this gene is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family.
Background References
1. Souter EA et al. Disruption of VGLUT1 in Cholinergic Medial Habenula Projections Increases Nicotine Self-Administration. eNeuro. 2022 Jan
2. Jin S et al. Molecular Profiling of VGluT1 AND VGluT2 Ventral Subiculum to Nucleus Accumbens Shell Projections. Neurochem Res. 2023 Aug
Subcellular Location
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cell membrane, Synapse, synaptosome.
Synonyms
BNPI antibody
Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
Brain specific Na dependent inorganic phosphate cotransporter antibody
Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody
Slc17a7 antibody
Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody
Solute carrier family 17 member 7 antibody
Vesicular glutamate transporter 1 antibody
VGLU1_HUMAN antibody
VGLUT 1 antibody
ExpandImages
-
This data was developed using HA601371, the same antibody clone in a different buffer formulation.
Western blot analysis of VGLUT1 on different lysates with Mouse anti-VGLUT1 antibody (HA601371) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat) (20 µg/Lane)
Lane 2: Rat brain tissue lysate (no heat) (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601371) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA601371, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Mouse anti-VGLUT1 antibody (HA601371) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601371) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601371, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-VGLUT1 antibody (HA601371) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601371) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601371, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Mouse anti-VGLUT1 antibody (HA601371) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601371) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601371, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-VGLUT1 antibody (HA601371) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601371) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"