SLC32A1 / VGAT Recombinant Mouse Monoclonal Antibody [PSH10-47] - BSA and Azide free
Catalog# HA610228
SLC32A1 / VGAT Recombinant Mouse Monoclonal Antibody [PSH10-47] - BSA and Azide free
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IHC-Fr
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IHC-P
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Mouse
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Rat
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HA601384
含抗保成分
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
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unconjugated
Overview
Product Name
SLC32A1 / VGAT Recombinant Mouse Monoclonal Antibody [PSH10-47] - BSA and Azide free
Antibody Type
Recombinant Mouse Monoclonal Antibody
Species Reactivity
Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
IHC-Fr, IHC-P
Target Molecular Weight
Predicted band size: 57 kDa
Positive Control
Mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue.
Conjugation
unconjugated
Clone Number
PSH10-47
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Vesicular inhibitory amino acid transporter is a protein that in humans is encoded by the SLC32A1 gene. The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II.
Background References
1. Heron SE et al. Association of SLC32A1 Missense Variants With Genetic Epilepsy With Febrile Seizures Plus. Neurology. 2021 May
2. Guerrini R et al. SLC32A1: One More Gene Contributing to the Solution of the Genetic Generalized Epilepsies Mystery. Neurology. 2021 May
Subcellular Location
Cytoplasmic vesicle membrane, Presynapse.
Synonyms
bA122O1.1 antibody
GABA and glycine transporter antibody
hVIAAT antibody
SLC32A 1 antibody
Slc32a1 antibody
solute carrier family 32 (GABA vesicular transporter) member 1 antibody
Solute carrier family 32 member 1 antibody
Vesicular GABA Amino Acid Transporter antibody
Vesicular GABA transporter antibody
Vesicular inhibitory amino acid transporter antibody
ExpandImages
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This data was developed using HA601384, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:500 (SLC32A1 / VGAT, HA601384, green); 1:500 (Parvalbumin, ET1703-15, red)
Antigen retrieval: Not required -
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This data was developed using HA601384, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601384) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601384, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601384) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601384, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601384) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601384, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601384) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"