Parvalbumin Recombinant Rabbit Monoclonal Antibody [JM100-08]
Catalog# ET1703-15
Parvalbumin Recombinant Rabbit Monoclonal Antibody [JM100-08]
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
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IP
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
Overview
Product Name
Parvalbumin Recombinant Rabbit Monoclonal Antibody [JM100-08]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Paravalbumin aa 1-110 / 110.
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IHC-Fr, IF-Tissue, IP
Molecular Weight
Predicted band size: 12 kDa
Positive Control
RPMI 8226 cell lysate, human kidney tissue, mouse kidney tissue, mouse cerebellum tissue, rat cerebellum tissue.
Conjugation
unconjugated
Clone Number
JM100-08
RRID
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:200-1:1,000
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IHC-Fr
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1:500-1:1,000
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IF-Tissue
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1:500
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IP
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1-2μg/sample
Target
Function
Parvalbumin (PV) is a calcium-binding protein with low molecular weight (typically 9-11 kDa). In humans, it is encoded by the PVALB gene. It is not a member of the albumin family; it is named for its size (parv-, from Latin parvus small) and its ability to coagulate. It has three EF hand motifs and is structurally related to calmodulin and troponin C. Parvalbumin is found in fast-contracting muscles, where its levels are highest, as well as in the brain and some endocrine tissues. Parvalbumin is a small, stable protein containing EF-hand type calcium binding sites. It is involved in calcium signaling. Calcium binding proteins like parvalbumin play a role in many physiological processes, namely cell-cycle regulation, second messenger production, muscle contraction, organization of microtubules and phototransduction. Therefore, calcium-binding proteins must distinguish calcium in the presence of high concentrations of other metal ions. The mechanism for the calcium selectivity has been extensively studied.
Background References
1. Cornez G et al. Anatomically discrete sex differences in neuroplasticity in zebra finches as reflected by perineuronal nets. PLoS One 10:e0123199 (2015).
2. Whissell PD et al. Comparative density of CCK- and PV-GABA cells within the cortex and hippocampus. Front Neuroanat 9:124 (2015).
Sequence Similarity
Belongs to the parvalbumin family.
Subcellular Location
Axon, cytoplasm, synapse.
Synonyms
D22S749 antibody
MGC116759 antibody
Parvalbumin alpha antibody
PRVA_HUMAN antibody
PVALB antibody
Images
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Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1/1,000
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-15) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-15) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Western blot analysis of Parvalbumin on different lysates with Rabbit anti-Parvalbumin antibody (ET1703-15) at 1/1,000 dilution.
Lane 1: RPMI 8226 cell lysate (20 µg/Lane)
Lane 2: SK-MEL-28 cell lysate (negative) (20 µg/Lane)
Lane 3: Mouse skeletal muscle tissue lysate (20 µg/Lane)
Lane 4: Mouse cerebellum tissue lysate (40 µg/Lane)
Lane 5: Rat cerebellum tissue lysate (40 µg/Lane)
Predicted band size: 12 kDa
Observed band size: 14 kDa
Exposure time: Lane 1-3: 30 seconds; Lane 4-5: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-15) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IF-tissue
Species: Mouse
Site: Cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
Parvalbumin was immunoprecipitated from 0.2 mg RPMI 8226 cell lysate with ET1703-15 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET1703-15 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: RPMI 8226 cell lysate (input)
Lane 2: ET1703-15 IP in RPMI 8226 cell lysate
Lane 3: Rabbit IgG instead of ET1703-15 in RPMI 8226 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 10 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"