Nestin Recombinant Antibody [PSH07-89] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
Catalog# HA610302
Nestin Recombinant Antibody [PSH07-89] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
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IHC-Fr
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WB
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Human
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Mouse
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Rat
Overview
Product Name
Nestin Recombinant Antibody [PSH07-89] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within mouse Nestin aa 700-1,000.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IHC-Fr, WB
Molecular Weight
Predicted band size: 207 kDa
Positive Control
RD cell lysate, C2C12 cell lysate, C6 cell lysate.
Conjugation
unconjugated
Clone Number
PSH07-89
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-Fr
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1:200
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WB
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1:10,000
Target
Function
Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or a-internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differen-tiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.
Background References
1. Chen H et al. Targeting Nestin(+) hepatic stellate cells ameliorates liver fibrosis by facilitating TbetaRI degradation. J Hepatol. 2021 May
2. Wang J et al. Nestin promotes pulmonary fibrosis via facilitating recycling of TGF-beta receptor I. Eur Respir J. 2022 May
Subcellular Location
Cytoplasm, Intermediate filament.
Synonyms
ESTM 46 antibody
FLJ 21841 antibody
FLJ21841 antibody
Intermediate filament protein antibody
Nbla00170 antibody
nes antibody
NEST_HUMAN antibody
Nestin antibody
Images
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Application: IHC-Fr
Species: Mouse
Site: embryonic cartilage
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Mouse
Site: kidney
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: kidney
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
☑ Relative expression (RE)
Western blot analysis of Nestin on different lysates with Guinea pig anti-Nestin antibody (HA610302) at 1/10,000 dilution.
Lane 1: RD cell lysate
Lane 2: 293T cell lysate (negative)
Lane 3: C2C12 cell lysate
Lane 4: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 207 kDa
Observed band size: 100-300 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610302) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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