QKI-6 Recombinant Antibody [PSH10-23] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
Overview
Product Name
QKI-6 Recombinant Antibody [PSH10-23] - Guinea pig IgG2 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Synthetic peptide within human QKI-6 aa 281-319 / 319.
Species Reactivity
Mouse, Rat
Validated Applications
IHC-Fr, WB
Molecular Weight
Predicted band size: 35 kDa
Positive Control
Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH10-23
Product Features
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
-
IHC-Fr
-
1:200
-
WB
-
1:5,000
Target
Function
Quaking homolog, KH domain RNA binding (mouse), also known as QKI, is a protein which in humans is encoded by the QKI gene. QKI belongs to a family of RNA-binding proteins called STAR proteins for Signal Transduction and Activation of RNA. They have an HNRNPK homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1) and SF1. Two more new members are KHDRBS3 and KHDRBS2. The QKI gene is implicated as being important in schizophrenia, and QKI controls translation of many oligodendrocyte-related genes.
Background References
1. Zhao Z et al. QKI shuttles internal m(7)G-modified transcripts into stress granules and modulates mRNA metabolism. Cell. 2023 Jul
2. Suh YY et al. MYB/MYBL1::QKI fusion-positive diffuse glioma. J Neuropathol Exp Neurol. 2023 Feb
Subcellular Location
Cytoplasm, cytosol, Nucleus.
Synonyms
DKFZp586I0923 antibody
HKQ antibody
Homolog of mouse quaking QKI KH domain RNA binding protein antibody
Hqk antibody
HQK1 antibody
HqkI antibody
OTTHUMP00000017581 antibody
OTTHUMP00000017582 antibody
OTTHUMP00000017583 antibody
Protein quaking antibody
ExpandImages
-
Application: IHC-Fr
Species: Mouse
Site: cerebellum
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
☑ Relative expression (RE)
Western blot analysis of QKI-6 on different lysates with Guinea pig anti-QKI-6 antibody (HA610332) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse kidney tissue lysate (negative)
Lane 3: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610332) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"