SOX9 Recombinant Antibody [SN74-09] - Rat IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610442
SOX9 Recombinant Antibody [SN74-09] - Rat IgG1 (Chimeric) - BSA and Azide free
-
IHC-P
-
Human
-
Mouse
-
Rat
-
HA601650
含抗保成分
-
unconjugated
Overview
Product Name
SOX9 Recombinant Antibody [SN74-09] - Rat IgG1 (Chimeric) - BSA and Azide free
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within human SOX9 aa 140-340.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IHC-P
Target Molecular Weight
Predicted band size: 56 kDa
Positive Control
Human Colon, Mouse Colon, Rat Colon.
Conjugation
unconjugated
Clone Number
SN74-09
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
SOX-9 recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It is expressed by proliferating but not hypertrophic chondrocytes that is essential for differentiation of precursor cells into chondrocytes and, with steroidogenic factor 1, regulates transcription of the anti-Müllerian hormone (AMH) gene. SOX-9 also plays a pivotal role in male sexual development; by working with Sf1, SOX-9 can produce AMH in Sertoli cells to inhibit the creation of a female reproductive system. It also interacts with a few other genes to promote the development of male sexual organs. The process starts when the transcription factor Testis determining factor (encoded by the sex-determining region SRY of the Y chromosome) activates SOX-9 activity by binding to an enhancer sequence upstream of the gene. Next, Sox9 activates FGF9 and forms feedforward loops with FGF9[ and PGD2. These loops are important for producing SOX-9; without these loops, SOX-9 would run out and the development of a female would almost certainly ensue. Activation of FGF9 by SOX-9 starts vital processes in male development, such as the creation of testis cords and the multiplication of Sertoli cells.
Background References
1. Aggarwal S et al. SOX9 switch links regeneration to fibrosis at the single-cell level in mammalian kidneys. Science. 2024 Feb
2. Liu Y et al. Yap-Sox9 signaling determines hepatocyte plasticity and lineage-specific hepatocarcinogenesis. J Hepatol. 2022 Mar
Subcellular Location
Nucleus.
Synonyms
campomelic dysplasia autosomal sex reversal antibody
CMD 1 antibody
CMD1 antibody
CMPD 1 antibody
CMPD1 antibody
SOX 9 antibody
Sox9 antibody
SOX9_HUMAN antibody
SRA 1 antibody
SRA1 antibody
ExpandImages
-
This data was developed using HA601650, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Colon
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601650, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature. -
This data was developed using HA601650, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Colon
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601650, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature. -
This data was developed using HA601650, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Colon
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601650, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1023, 30 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
SOX9 Recombinant Rabbit Monoclonal Antibody [SN74-09]
Application: WB,IF-Cell,IHC-P,IP,FC,CUT&Tag-seq,mIHC
Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
Conjugate: unconjugated
SOX9 Recombinant Antibody [SN74-09] - Mouse IgG1 (Chimeric) - BSA and Azide free
Application: IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
SOX9 Recombinant Antibody [SN74-09] - Mouse IgG1 (Chimeric)
Application: IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
SOX9 Recombinant Antibody - Mouse IgG1 (Chimeric)
Application: mIHC
Reactivity: Human
Conjugate: unconjugated
SOX9 Recombinant Rabbit Monoclonal Antibody [SN74-09] - BSA and Azide free
Application: WB,IF-Cell,IHC-P,IP,FC,CUT&Tag-seq
Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
Conjugate: unconjugated
SOX9 Recombinant Rabbit Monoclonal Antibody
Application: mIHC
Reactivity: Human
Conjugate: unconjugated
SOX9 Recombinant Antibody - Rat IgG1 (Chimeric)
Application: mIHC
Reactivity: Human
Conjugate: unconjugated
SOX9 Recombinant Antibody [SN74-09] - Rat IgG1 (Chimeric)
Application: IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
SOX9 Recombinant Rabbit Monoclonal Antibody [PSH13-59] - BSA and Azide free
Application: IHC-Fr,IHC-P,WB,IF-Cell,IP,ChIP,FC(Intra)
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
