SOX9 Recombinant Rabbit Monoclonal Antibody [PSH13-59]
Catalog# HA723548
SOX9 Recombinant Rabbit Monoclonal Antibody [PSH13-59]
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IHC-Fr
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IHC-P
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WB
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IF-Cell
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IP
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Human
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Mouse
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Rat
Overview
Product Name
SOX9 Recombinant Rabbit Monoclonal Antibody [PSH13-59]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within mouse SOX9 aa 1-450.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IHC-Fr, IHC-P, WB, IF-Cell, IP
Molecular Weight
Predicted band size: 56 kDa
Positive Control
Human colon tissue, mouse brain tissue, mouse colon tissue, rat brain tissue, rat colon tissue, NIH/3T3 cell lysate, PANC-1 cell lysate, SW480 cell lysate, MDA-MB-231 cell lysate, NIH/3T3.
Conjugation
unconjugated
Clone Number
PSH13-59
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-Fr
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1:500
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IHC-P
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1:1,000
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WB
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1:5,000
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IF-Cell
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1:100
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IP
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1-2μg/sample
Target
Function
SOX-9 recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It is expressed by proliferating but not hypertrophic chondrocytes that is essential for differentiation of precursor cells into chondrocytes and, with steroidogenic factor 1, regulates transcription of the anti-Müllerian hormone (AMH) gene. SOX-9 also plays a pivotal role in male sexual development; by working with Sf1, SOX-9 can produce AMH in Sertoli cells to inhibit the creation of a female reproductive system. It also interacts with a few other genes to promote the development of male sexual organs. The process starts when the transcription factor Testis determining factor (encoded by the sex-determining region SRY of the Y chromosome) activates SOX-9 activity by binding to an enhancer sequence upstream of the gene. Next, Sox9 activates FGF9 and forms feedforward loops with FGF9[ and PGD2. These loops are important for producing SOX-9; without these loops, SOX-9 would run out and the development of a female would almost certainly ensue. Activation of FGF9 by SOX-9 starts vital processes in male development, such as the creation of testis cords and the multiplication of Sertoli cells.
Background References
1. Aggarwal S et al. SOX9 switch links regeneration to fibrosis at the single-cell level in mammalian kidneys. Science. 2024 Feb
2. Liu Y et al. Yap-Sox9 signaling determines hepatocyte plasticity and lineage-specific hepatocarcinogenesis. J Hepatol. 2022 Mar
Subcellular Location
Nucleus.
Synonyms
campomelic dysplasia autosomal sex reversal antibody
CMD 1 antibody
CMD1 antibody
CMPD 1 antibody
CMPD1 antibody
SOX 9 antibody
Sox9 antibody
SOX9_HUMAN antibody
SRA 1 antibody
SRA1 antibody
ExpandImages
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Application: IHC-Fr
Species: Mouse
Site: brain
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: brain
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-SOX9 antibody (HA723548) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723548) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-SOX9 antibody (HA723548) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723548) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-SOX9 antibody (HA723548) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723548) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SOX9 antibody (HA723548) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723548) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-SOX9 antibody (HA723548) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723548) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Western blot analysis of SOX9 on different lysates with Rabbit anti-SOX9 antibody (HA723548) at 1/5,000 dilution.
Lane 1: NIH/3T3 cell lysate
Lane 2: PANC-1 cell lysate
Lane 3: SW480 cell lysate
Lane 4: MDA-MB-231 cell lysate
Lane 5: MCF7 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 70 kDa
Exposure time: 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723548) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of NIH/3T3 cells labeling SOX9 with Rabbit anti-SOX9 antibody (HA723548) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SOX9 antibody (HA723548) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
SOX9 was immunoprecipitated from 0.2 mg MDA-MB-231 cell lysate with HA723548 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723548 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: MDA-MB-231 cell lysate (input)
Lane 2: HA723548 IP in MDA-MB-231 cell lysate
Lane 3: Rabbit IgG instead of HA723548 in MDA-MB-231 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 46 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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