Phospho-Tau (T217) Recombinant Rabbit Monoclonal Antibody [PSH16-75] - BSA and Azide free (Capture)
Overview
Product Name
Phospho-Tau (T217) Recombinant Rabbit Monoclonal Antibody [PSH16-75] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Thr217 of Phospho-Tau (T217).
Species Reactivity
Human
Validated Applications
ELISA(Cap)
Positive Control
Recombinant Human Phospho-Tau (T217) protein (HA211033).
Conjugation
unconjugated
Clone Number
PSH16-75
Product Features
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH10-42] to Tau antibody (Detector) (HA723209) and Recombinant Human Phospho-Tau (T217) protein (HA211033) as the standard. The reference range value is 58-15,000 pg/mL.
Target
Function
Tau, also known as MAPT (microtubule-associated protein tau), MAPTL, MTBT1 or TAU, is a 758 amino acid protein that localizes to the cytoplasm, as well as to the cytoskeleton and the cell membrane, and contains four Tau/MAP repeats. Expressed in neuronal tissue and existing as multiple alternatively spliced isoforms, Tau functions to promote microtubule assembly and stability and is thought to be involved in the maintenance of neuronal polarity. Tau may also link microtubules with neural plasma membrane components and, addition to its role in microtubule stability, is also necessary for cytoskeletal plasticity. Tau is highly subject to a variety of post-translational modifications, including phosphorylation on serine and threonine residues, polyubiquitination (and subsequent proteasomal degradation) and glycation of specific Tau isoforms. Defects in the gene encoding Tau are associated with Alzheimers disease, pallido-ponto-nigral degeneration (PPND), corticobasal degeneration (CBD) and progressive supranuclear palsy (PSP).
Background References
1. Bayoumy S, Verberk IMW, den Dulk B, et al. Clinical and analytical comparison of six Simoa assays for plasma P-tau isoforms P-tau181, P-tau217, and P-tau231. Alzheimers Res Ther. 2021;13(1):198. Published 2021 Dec 4. doi:10.1186/s13195-021-00939-9
2. Janelidze S, Berron D, Smith R, et al. Associations of Plasma Phospho-Tau217 Levels With Tau Positron Emission Tomography in Early Alzheimer Disease. JAMA Neurol. 2021;78(2):149-156. doi:10.1001/jamaneurol.2020.4201
Subcellular Location
Cell membrane. Cytoplasm. Secreted.
UNIPROT
Synonyms
AI413597 antibody
AW045860 antibody
DDPAC antibody
FLJ31424 antibody
FTDP 17 antibody
G protein beta1/gamma2 subunit interacting factor 1 antibody
MAPT antibody
MAPTL antibody
MGC134287 antibody
MGC138549 antibody
ExpandImages
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![Sandwich ELISA analysis of Human Tau (phospho T217) matched pair antibodies<br /><br />Capture: <a href="/products/HA723877" style="font-weight: bold;text-decoration: underline;">HA723877</a>, Phospho-Tau (T217) Rabbit mAb [PSH16-75]<br />Detector: <a href="/products/HA723209" style="font-weight: bold;text-decoration: underline;">HA723209</a>, Phospho-Tau (T217) Rabbit mAb [PSH10-42]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723877" style="font-weight: bold;text-decoration: underline;">HA723877</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA211033) starting from 15000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723209" style="font-weight: bold;text-decoration: underline;">HA723209</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723877_1.png?v=20250809115500)
Sandwich ELISA analysis of Human Tau (phospho T217) matched pair antibodies
Capture: HA723877, Phospho-Tau (T217) Rabbit mAb [PSH16-75]
Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723877) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA211033) starting from 15000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of Tau (phospho T217) in <br />293T+OE-human Tau441 2N4R and 293T+OE-Vector extract samples based on a 1000 µg/ml extract load.<br />293T+OE-human Tau441 2N4R represented the overexpression of Tau (phospho T217) protein. 293T+OE-Vector represented the non-expression of Tau (phospho T217) protein.<br /><br />Capture: <a href="/products/HA723877" style="font-weight: bold;text-decoration: underline;">HA723877</a>, Phospho-Tau (T217) Rabbit mAb [PSH16-75]<br />Detector: <a href="/products/HA723209" style="font-weight: bold;text-decoration: underline;">HA723209</a>, Phospho-Tau (T217) Rabbit mAb [PSH10-42]<br /><br />Interpolated concentration of Tau (phospho T217) was measured in duplicate at different sample concentrations and interpolated from the Tau (phospho T217) standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Tau (phospho T217) concentration was determined to be 258 ug/mL in 293T+OE-human Tau441 2N4R cell extract. There was no detectable signal in 293T+OE-Vector cell extract.](https://storage.huabio.cn/huabio/productImg/HA723877_2.png?v=20250809115522)
Interpolated concentrations of Tau (phospho T217) in
293T+OE-human Tau441 2N4R and 293T+OE-Vector extract samples based on a 1000 µg/ml extract load.
293T+OE-human Tau441 2N4R represented the overexpression of Tau (phospho T217) protein. 293T+OE-Vector represented the non-expression of Tau (phospho T217) protein.
Capture: HA723877, Phospho-Tau (T217) Rabbit mAb [PSH16-75]
Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42]
Interpolated concentration of Tau (phospho T217) was measured in duplicate at different sample concentrations and interpolated from the Tau (phospho T217) standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Tau (phospho T217) concentration was determined to be 258 ug/mL in 293T+OE-human Tau441 2N4R cell extract. There was no detectable signal in 293T+OE-Vector cell extract.
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