HRP Conjugated beta Actin Recombinant Rabbit Monoclonal Antibody [PSH03-63] - Loading control
Catalog# HA724235H
HRP Conjugated beta Actin Recombinant Rabbit Monoclonal Antibody [PSH03-63] - Loading control
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WB
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Human
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Mouse
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Rat
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Pig
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Monkey
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HRP
Overview
Product Name
HRP Conjugated beta Actin Recombinant Rabbit Monoclonal Antibody [PSH03-63] - Loading control
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within N-terminal residues of β-Actin.
Species Reactivity
Human, Mouse, Rat, Pig, Monkey
Validated Applications
WB
Molecular Weight
Predicted band size: 42 kDa
Positive Control
Mouse brain tissue lysate, Mouse liver tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate, Pig brain tissue lysate, HeLa cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, COS-1 cell lysate.
Conjugation
HRP
Clone Number
PSH03-63
Product Features
Form
Liquid
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000-1:5,000
Target
Function
Actins are highly conserved proteins involved in cell motility, structure and integrity. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Beta actin is usually used as a loading control, for among others, the integrity of cells, protein degradation, in Western Blotting.
Background References
1. Ponte P., Ng S.Y., Engel J., Gunning P., Kedes L."Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA." Nucleic Acids Res. 12:1687-1696(1984) Ohmori H., Toyama S., Toyama S."Direct proof that the primary site of action of cytochalasin on cell motility processes is actin."J. Cell Biol. 116:933-941(1992)
Subcellular Location
Cytoskeleton.
Synonyms
A26C1A antibody
A26C1B antibody
ACTB antibody
ACTB_HUMAN antibody
Actin beta antibody
Actin cytoplasmic 1 antibody
Actin, cytoplasmic 1, N-terminally processed antibody
Actx antibody
b actin antibody
Beta cytoskeletal actin antibody
ExpandImages
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Western blot analysis of beta Actin on different lysates with Rabbit anti-beta Actin antibody (HA724235H) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat liver tissue lysate
Lane 4: Pig brain tissue lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724235H, HRP) at 1/2,000 dilution was used in primary antibody dilution buffer (K1803) at room temperature for 1 hour. -
Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (HA724235H) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: C6 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724235H, HRP) at 1/2,000 dilution was used in primary antibody dilution buffer (K1803) at room temperature for 1 hour.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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