HRP Conjugated Human FAP Recombinant Rabbit Monoclonal Antibody [PSH26-56] - Detector
Overview
Product Name
HRP Conjugated Human FAP Recombinant Rabbit Monoclonal Antibody [PSH26-56] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human FAP aa 26-760 (HA211419).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human FAP protein (HA211419).
Conjugation
HRP
Clone Number
PSH26-56
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH26-55] to Human FAP (Capture) (HA725499) and Recombinant Human FAP protein (HA211419) as the standard. The reference range value is 156.3-20,000 pg/mL.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
FAP (fibroblast activation protein) is a cell surface glycoprotein and serine protease that is expressed primarily in fetal mesenchymal tissues and epithelial cancer fibroblasts. In cancer, FAP functions to promote cellular proliferation. In embryonic development, FAP functions to remodel developing tissues. FAP acts as an integral membrane gelatinase composed of N-glycosylated proteolytically inactive subunits. FAP expression on chondrocyte membranes is upregulated by the combination of the cytokines IL-1 and OSM and has been shown to increase in osteoarthritic patients. This expression is co-localized with MMP-1and MMP-13 as well as CD44 (variants v3 and v7/8). Mice that lack all copies of the FAP gene have been found to be fertile and to have developmental defects or change in cancer susceptibility.
Background References
1. Jia, B. et al. 2016. GPR30 Promotes Prostate Stromal Cell Activation via Suppression of ERα Expression and Its Downstream Signaling Pathway. Endocrinology. 157: 3023-35.
2. Knopf, JD. et al. 2015. The stromal cell-surface protease fibroblast activation protein-α localizes to lipid rafts and is recruited to invadopodia. Biochim. Biophys. Acta. 1853: 2515-25.
Subcellular Location
Cell membrane. Cell surface.
UNIPROT
Synonyms
170 kDa melanoma membrane bound gelatinase antibody
170 kDa melanoma membrane-bound gelatinase antibody
DPPIV antibody
FAP antibody
FAPA antibody
Fibroblast activation protein alpha antibody
Integral membrane serine protease antibody
SEPR_HUMAN antibody
Seprase antibody
Images
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![Sandwich ELISA analysis of Human FAP matched pair antibodies<br /><br />Capture: <a href="/products/HA725499" style="font-weight: bold;text-decoration: underline;">HA725499</a>, Human FAP Rabbit mAb [PSH26-55]<br />Detector: <a href="/products/HA725500" style="font-weight: bold;text-decoration: underline;">HA725500</a>, Human FAP Rabbit mAb [PSH26-56]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA725499" style="font-weight: bold;text-decoration: underline;">HA725499</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human FAP protein (<a href="/products/HA211419" style="font-weight: bold;text-decoration: underline;">HA211419</a>) starting from 20,000 pg/mL to 0 pg/mL and detect antibody (<a href="/products/HA725500" style="font-weight: bold;text-decoration: underline;">HA725500</a>, HRP, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA725500H_1.jpg?v=20260908104421)
Sandwich ELISA analysis of Human FAP matched pair antibodies
Capture: HA725499, Human FAP Rabbit mAb [PSH26-55]
Detector: HA725500, Human FAP Rabbit mAb [PSH26-56]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725499) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human FAP protein (HA211419) starting from 20,000 pg/mL to 0 pg/mL and detect antibody (HA725500, HRP, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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