Calbindin Recombinant Rabbit Monoclonal Antibody [JF05-01] - BSA and Azide free
Catalog# HA750351
Calbindin Recombinant Rabbit Monoclonal Antibody [JF05-01] - BSA and Azide free
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WB
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IF-Tissue
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IHC-P
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IHC-Fr
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Mouse
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Rat
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ET1702-54
含抗保成分
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
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unconjugated
Overview
Product Name
Calbindin Recombinant Rabbit Monoclonal Antibody [JF05-01] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Calbindin aa 51-100 / 261.
Species Reactivity
Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IF-Tissue, IHC-P, IHC-Fr
Target Molecular Weight
Predicted band size: 30 kDa
Positive Control
Mouse brain tissue lysate, rat brain tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, human kidney tissue, mouse cerebellum tissue, PC-12, 293T, rat brain tissue, mouse brain tissue, mouse kidney tissue.
Conjugation
unconjugated
Clone Number
JF05-01
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Calbindin 1 is a protein that in humans is encoded by the CALB1 gene. It belongs to the calbindin family of calcium-binding proteins, along with calretinin (CALB2). The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease.
Background References
1. Noble JW et al. Exploring Calbindin-IMPase fusion proteins structure and activity. Biochem Biophys Rep. 2022 May
2. Attig J et al. Human endogenous retrovirus onco-exaptation counters cancer cell senescence through calbindin. J Clin Invest. 2023 Jul
Sequence Similarity
Belongs to the calbindin family.
Subcellular Location
Cytosol, Nucleus.
Synonyms
avian-type antibody
CAB27 antibody
CALB 1 antibody
CALB antibody
CALB1 antibody
CALB1_HUMAN antibody
Calbindin 1 28kDa antibody
Calbindin antibody
Calbindin D28 antibody
D 28K antibody
ExpandImages
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This data was developed using ET1702-54, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1/2,000
Antigen retrieval: Not required -
This data was developed using ET1702-54, the same antibody clone in a different buffer formulation.
Application: IF-tissue
Species: Mouse
Site: Cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
This data was developed using ET1702-54, the same antibody clone in a different buffer formulation.
Application: IF-tissue
Species: Rat
Site: Cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
This data was developed using ET1702-54, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Calbindin antibody (ET1702-54) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-54) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1702-54, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Calbindin antibody (ET1702-54) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-54) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
This data was developed using ET1702-54, the same antibody clone in a different buffer formulation.
Western blot analysis of Calbindin on different lysates with Rabbit anti-Calbindin antibody (ET1702-54) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (negative) (20 µg/Lane)
Lane 3: Rat brain tissue lysate (20 µg/Lane)
Lane 4: C6 cell lysate (negative) (20 µg/Lane)
Lane 5: Mouse kidney tissue lysate (20 µg/Lane)
Lane 6: Rat kidney tissue lysate (20 µg/Lane)
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-54) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"