TBR1 Recombinant Rabbit Monoclonal Antibody [JF10-00] - BSA and Azide free
Catalog# HA750366
TBR1 Recombinant Rabbit Monoclonal Antibody [JF10-00] - BSA and Azide free
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WB
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IHC-P
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IF-Tissue
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IHC-Fr
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Human
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Mouse
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Rat
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ET1702-97
含抗保成分
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
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unconjugated
Overview
Product Name
TBR1 Recombinant Rabbit Monoclonal Antibody [JF10-00] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human TBR1 aa 30-75 / 682.
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IF-Tissue, IHC-Fr
Target Molecular Weight
Predicted band size: 74 kDa
Positive Control
Human brain tissue lysates, mouse brain tissue lysates, rat brain tissue lysates, human brain tissue, mouse brain tissue, rat brain tissue, mouse hippocampus tissue, rat hippocampus tissue, E14.5 mouse embryonic brain tissue.
Conjugation
unconjugated
Clone Number
JF10-00
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
A novel murine and human gene, TBR-1, encodes a putative transcription factor related to the Brachyrury (T) gene that is expressed only in postmitotic cells. T-brain-1 (TBR-1) mRNA is largely restricted to the cerebral cortex, where, during embryogenesis, it defines different regions that give rise to the palecortex, limbic cortex and neocortex. TBR-1, Pax-6 and Emx-1 are expressed in the mouse and chicken pallium. The pallio-subpallial boundary lies at the interface between the TBR-1 and Dlx-2 expression domains. Chicken genes homolgous to these mouse genes are expressed in topologically comparable patterns during development, suggesting that mouse and chicken may have similar histogenetic specification processes and field homologies. CASK/LIN-2, a membrane-associated guanylate kinase, is required for EGFR localization and signaling. In adult rat brain, CASK is concentrated at neuronal synapses and binds to the cell-surface proteins. CASK can interact with TBR-1, which is involved in forebrain development. CASK enters into the nucleus and binds to a specific DNA sequence (the T-element) in a complex with TBR-1. Thus, CASK acts as a coactivator of TBR-1 to induce transcription of T-element containing genes, including reelin.
Background References
1. Crespo I et al. Tbr1 Misexpression Alters Neuronal Development in the Cerebral Cortex. Mol Neurobiol. 2022 Sep
2. Sollis E et al. Characterization of the TBR1 interactome: variants associated with neurodevelopmental disorders disrupt novel protein interactions. Hum Mol Genet. 2023 Apr
Tissue Specificity
Brain.
Subcellular Location
Nucleus.
Synonyms
T box brain 1 antibody
T box brain protein 1 antibody
T brain 1 protein antibody
T-box brain protein 1 antibody
T-brain-1 antibody
TBR 1 antibody
TBR-1 antibody
Tbr1 antibody
TBR1_HUMAN antibody
TES 56 antibody
ExpandImages
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This data was developed using ET1702-97, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex (P0)
Sample: Frozen section
Antibody concentration: 1:1,000
Antigen retrieval: Not required -
This data was developed using ET1702-97, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: E14.5 embryonic brain
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required -
This data was developed using ET1702-97, the same antibody clone in a different buffer formulation.
Application: IF-tissue
Species: Mouse
Site: E14.5 embryonic brain
Sample: Paraffin-embedded section
Antibody concentration: 1:500 -
This data was developed using ET1702-97, the same antibody clone in a different buffer formulation.
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling TBR1 with Rabbit anti-TBR1 antibody (ET1702-97) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-97, green) at 1/500 dilution overnight at 4 ℃, washed with PBS.
Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
This data was developed using ET1702-97, the same antibody clone in a different buffer formulation.
Western blot analysis of TBR1 on different lysates with Rabbit anti-TBR1 antibody (ET1702-97) at 1/1,000 dilution.
Lane 1: Human brain tissue lysate (40 µg/Lane)
Lane 2: Mouse brain tissue lysate (40 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-97) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1702-97, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-TBR1 antibody (ET1702-97) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-97) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1702-97, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-TBR1 antibody (ET1702-97) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-97) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1702-97, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-TBR1 antibody (ET1702-97) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-97) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1702-97, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-TBR1 antibody (ET1702-97) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-97) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1702-97, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-TBR1 antibody (ET1702-97) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-97) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1702-97, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-TBR1 antibody (ET1702-97) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-97) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"