ADAM17 Recombinant Rabbit Monoclonal Antibody [JM10-35] - BSA and Azide free
Catalog# HA750370
ADAM17 Recombinant Rabbit Monoclonal Antibody [JM10-35] - BSA and Azide free
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WB
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IF-Cell
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IF-Tissue
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FC
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IP
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IHC-Fr
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IHC-P
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
Predicted species support after-sales service
Overview
Product Name
ADAM17 Recombinant Rabbit Monoclonal Antibody [JM10-35] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human ADAM17 aa 757-790 / 824.
Species Reactivity
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
Predicted species support after-sales service
Validated Applications
WB, IF-Cell, IF-Tissue, FC, IP, IHC-Fr, IHC-P
Molecular Weight
Predicted band size: 93 kDa
Positive Control
SW480 cell lysate, HepG2 cell lysate, HepG2, SKOV-3, SW480, Hela, mouse cerebral cortex tissue, mouse hippocampus tissue, rat hippocampus tissue.
Conjugation
unconjugated
Clone Number
JM10-35
Product Features
Form
Liquid
Concentration
1ug/ul
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100-1:200
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FC
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1:500-1:1,000
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IF-Tissue
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1:200-1:500
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IP
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Use at an assay dependent concentration.
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IHC-Fr
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1:100
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IHC-P
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1:500
Target
Function
A disintegrin and metalloprotease 17 (ADAM17), also called TACE (tumor necrosis factor-α-converting enzyme), is an 824-amino acid polypeptide that belongs to the ADAM protein family of disintegrins and metalloproteases. ADAM17 is understood to be involved in the processing of tumor necrosis factor alpha (TNF-α) at the surface of the cell, and from within the intracellular membranes of the trans-Golgi network. This process, which is also known as 'shedding', involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-α), and is of known physiological importance. ADAM17 was the first 'sheddase' to be identified, and is also understood to play a role in the release of a diverse variety of membrane-anchored cytokines, cell adhesion molecules, receptors, ligands, and enzymes.
Background References
1. Corbett GT et al. Activation of peroxisome proliferator-activated receptor a stimulates ADAM10-mediated proteolysis of APP. Proc Natl Acad Sci U S A 112:8445-50 (2015).
2. McClurg UL et al. Epithelial cell ADAM17 activation by Helicobacter pylori: role of ADAM17 C-terminus and Threonine-735 phosphorylation. Microbes Infect 17:205-14 (2015).
Tissue Specificity
Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
Post-translational Modification
The precursor is cleaved by a furin endopeptidase.; Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. Phosphorylation at THR-735 by MAPK14 is required for ADAM17-mediated ectodomain shedding.
Subcellular Location
Membrane.
Synonyms
A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
A disintegrin and metalloproteinase domain 17 antibody
ADA17_HUMAN antibody
ADAM 17 antibody
ADAM metallopeptidase domain 17 antibody
ADAM17 antibody
ADAM17 protein antibody
CD 156b antibody
CD156b antibody
CD156b antigen antibody
ExpandImages
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Western blot analysis of ADAM17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750370, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SW480 cell lysate
Lane 2: HepG2 cell lysate -
ICC staining of ADAM17 in HepG2 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750370, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Flow cytometric analysis of ADAM17 was done on HeLa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA750370, 1/1,000) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/200
Antigen retrieval: Not required -
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-ADAM17 antibody (HA750370) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750370) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-ADAM17 antibody (HA750370) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750370) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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